Molecular structure of E-64, CAS Number: 66701-25-5
E-64 (CAS 66701-25-5)
See product citations (14)
Alternate Names:
E-64 is also known as Proteinase Inhibitor E 64.
Application:
E-64 is an irreversible, potent, and highly selective broad spectrum cysteine proteinase and calpain activation inhibitor.
CAS Number:
66701-25-5
Molecular Weight:
357.40
Molecular Formula:
C15H27N5O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
E-64 is a compound recognized for its potent, irreversible inhibition of cysteine proteases. This characteristic makes it useful in biochemical research, particularly in the study of protease function and regulation. By inhibiting enzymes such as cathepsins and calpains, E-64 helps elucidate their roles in processes like protein degradation, cell cycle progression, and apoptosis. Researchers also use E-64 to explore the mechanisms of conditions where cysteine proteases are implicated. Its specificity for cysteine proteases also allows for the investigation of these enzymes in complex biological systems, contributing to a deeper understanding of proteolytic pathways. Moreover, E-64′s action in inhibiting protease activity is employed in the study of immune responses, where proteases play a role in antigen processing and presentation. In cell biology, E-64 can help dissect the contribution of cysteine proteases to intracellular protein trafficking and turnover.
E-64 (CAS 66701-25-5) References
- An intracellular form of cathepsin B contributes to invasiveness in cancer. | Szpaderska, AM. and Frankfater, A. 2001. Cancer Res. 61: 3493-500. PMID: 11309313
- Plasmodium falciparum: differential sensitivity in vitro to E-64 (cysteine protease inhibitor) and Pepstatin A (aspartyl protease inhibitor). | Bailly, E., et al. 1992. J Protozool. 39: 593-9. PMID: 1522541
- Inhibitors of calpain activation (PD150606 and E-64) and renal ischemia-reperfusion injury. | Chatterjee, PK., et al. 2005. Biochem Pharmacol. 69: 1121-31. PMID: 15763548
- Antiparasitic activity of cystine protease inhibitor E-64 against Giardia lamblia excystation in vitro and in vivo. | Hussein, EM., et al. 2009. J Egypt Soc Parasitol. 39: 111-9. PMID: 19530614
- Role of the cysteine protease interpain A of Prevotella intermedia in breakdown and release of haem from haemoglobin. | Byrne, DP., et al. 2009. Biochem J. 425: 257-64. PMID: 19814715
- Conformational mobility of active and E-64-inhibited actinidin. | Grozdanović, MM., et al. 2013. Biochim Biophys Acta. 1830: 4790-9. PMID: 23803410
- A cathepsin B inhibitor, E-64, improves the preimplantation development of bovine somatic cell nuclear transfer embryos. | Min, SH., et al. 2014. J Reprod Dev. 60: 21-7. PMID: 24240170
- In vitro antigiardial activity of the cysteine protease inhibitor E-64. | Carvalho, TB., et al. 2014. Rev Inst Med Trop Sao Paulo. 56: 43-7. PMID: 24553607
- Papain and its inhibitor E-64 reduce camelid semen viscosity without impairing sperm function and improve post-thaw motility rates. | Kershaw, CM., et al. 2017. Reprod Fertil Dev. 29: 1107-1114. PMID: 27156102
- Differential cathepsin responses to inhibitor-induced feedback: E-64 and cystatin C elevate active cathepsin S and suppress active cathepsin L in breast cancer cells. | Wilder, CL., et al. 2016. Int J Biochem Cell Biol. 79: 199-208. PMID: 27592448
- Efficient synthetic method for ethyl (+)-(2S,3S)-3-[(S)-3-methyl- 1-(3-methylbutylcarbamoyl)butylcarbamoyl]-2-oxiranecarb oxylate (EST), a new inhibitor of cysteine proteinases. | Tamai, M., et al. 1987. Chem Pharm Bull (Tokyo). 35: 1098-104. PMID: 3301019
- Small molecule inhibitor E-64 exhibiting the activity against African swine fever virus pS273R. | Liu, B., et al. 2021. Bioorg Med Chem. 35: 116055. PMID: 33607487
- Effect of E-64, a thiol protease inhibitor, on antibody formation in mice. | Amamoto, T., et al. 1984. Biochem Biophys Res Commun. 118: 117-23. PMID: 6696749
- Inhibitions of cathepsin B and cathepsin L by E-64 in vivo. II. Incorporation of [3H]E-64 into rat liver lysosomes in vivo. | Hashida, S., et al. 1982. J Biochem. 91: 1373-80. PMID: 7096292
- Bax cleavage is mediated by calpain during drug-induced apoptosis. | Wood, DE., et al. 1998. Oncogene. 17: 1069-78. PMID: 9764817
Inhibitor of:
α2-Macroglobulin, α2ML1, β3Gn-T6, 2310051M13Rik, 5330417C22Rik, 5430419D17Rik, 8030411F24Rik, A430107O13Rik, ACP2, AGA, Antipain dihydrochloride, apoL10a, apoL10b, apoL7a, apoL7c, apoL7e, ARRDC5, Arylsulfatase H, ASTL, ATF-6, Atg4a, BC048546, BLM hydrolase, BLOS3, BTBD7, C1q-A, C1rb, C1rL, C1s, Calpain, Calpain 15_Solh, Calpain 2, Calpain II, CAP2, Cathepsin, cathepsin 8, cathepsin A, cathepsin B, cathepsin D, cathepsin H, cathepsin J, cathepsin K, cathepsin L, cathepsin M, cathepsin Q, cathepsin S, CCZ1, Chondroitinase, Chymostatin, Chymotrypsin, CLAP, CLC-7, Cln1, CLN5, CLPSL1, CMTM3, Corin, CPA1, CPA2, CPA5, CPAMD8, CPD, CPVL, CRBP IV, CST, Cst9, CSTL1, CTLA-2α, Cyclophilin J, cystatin 10, cystatin 12, cystatin 13, cystatin 8, cystatin 9, cystatin B, cystatin C, Cystatin D, cystatin E/M, cystatin F, cystatin SN, Cytokeratin 14, D030074E01Rik, Desc4, DET1, Ear4, EG408191, EG433016, EG436523, EG622976, elastin, EMID1, ENSMUSG00000071517, ERAP1, ERp27, Esophagin, Expansin, FAM162B, FIBCD1, ficolin B, FYCO1, GalNAc-T7, Gm104, Gm46, GNPTG, GNS, Granzyme, granzyme A, granzyme C, granzyme D, granzyme E, granzyme F, granzyme G, granzyme H, granzyme M, granzyme N, H2-Aa, H2-Q1, H2-T10, H2-T9, HKIB9, HLA-B7, hole, HPS-6, HtrA2, HtrA4, ITM2B, Jade-1, Kex2, Kininogen II, KLHDC7A, Klk1b4, Klk26, KLKb2, KRTAP9-L2, LAMP-1, LAMP-2, Legumain, LOC150935, LOC727972, LOC728379, LOC728393, LOC728400, LONRF2, LPPR4, LRRC42, LRRC43, Lysozyme C, LYZL1, MAP LC3β, MAP LC3γ, Marapsin 2, Mast Cell Protease, Mast Cell Protease 5, Mast Cell Protease 8, Mast Cell Tryptase, Matriptase, Matriptase-2, mCST E2, megsin, Meprin A alpha, Mesotrypsinogen, MRCL3, MUP2, MUP3, MUP4, murinoglobulin 2, NAGPA, neuroserpin, Neurotrypsin, Olfr51, ORAOV1, OTTMUSG00000013284, OTTMUSG00000014862, OTTMUSG00000015173, OTTMUSG00000015220, OTTMUSG00000015279, OTTMUSG00000015337, OTTMUSG00000015417, OTTMUSG00000015643, OTTMUSG00000015752, OTTMUSG00000016293, OVCA2, P-Rex2, Papain, PC5/6, PCSK1N, PDIA2, PGPEP1L, PKC ζII, PLEKHM2, PNAd, Polyserase-1, POP2, PPIAL4, PPIAL4F, Ppil3, PPIL6, PQLC1, PRB1-3, PRB2, PRB3, prochymosin, Pronase, Prostasin, Protease, PRSS16, Prss28, Prss29, Prss32, PRSS33, Prss34, PRSS37, Prss39, Prss47, PRSS50, PRSS51, PTAG, Rab L5, Ran BP-10, RHBDD1, RHBDD2, RHBDL1, RHBDL2, Rhomboid, RNF181, S2P, SCGB1D4, SEC11A, Sel-1L3, Selenoprotein O, Semenogelin-2, SerpinA11, SerpinA1d, SerpinA2, SerpinA3g, SerpinA3j, SerpinA3n, SerpinB1b, Serpinb3c, Serpinb3d, SerpinB6B, SerpinB6C, Serpinb6d, SerpinB6e, SerpinB8, SerpinB9g, SERPINE3, Sg V, SPCS3, spectrin β II, Spi10, Spi11, SPINK10, SPINK14, SPINK2, SPINK3, SPINK6, SPINK7, SPPL2b, SPUVE, Staf-50, stefin A1L1, stefin A2, stefin A2l1, stefin A3L1, Svs7, Taspase 1, TATDN1, TATI, TECPR1, TESP3, Testisin, TGE Virus, TIP120, TLCK, TLR13, TM9SF1, TMEM169, TMEM41A, TMEM45B, TMPRSS11A, TMPRSS11B, TMPRSS11E, TMPRSS11F, TMPRSS12, TMPRSS13, TMPRSS3, TMPRSS5, TMX3, TPCK, TRAP, Trim29, TRIM43, TRIM43B, TRIM49B, TRIM50, TRIM56, TRIM6, TRIM64C, TRIM79, Trypsin-10, Trypsin-3, Trypsin-5, Tryptase γ, TSHZ2, TSP50, UBAP1, UBE2CBP, uPA, USP26, USP50, V-ATPase D2, V3R 2, Vmn2r50, Vmn2r62, Vmn2r65, Vmn2r70, Vrho, WBSCR17, WDFY2, WFDC17, WIPI-1, ZFP30, ZNF228, ZNF506, ZP4, and ZUFSP.Activator of:
2010005H15Rik, 2310051M13Rik, Acrosin, Aminopeptidase B, APEH, BC100530, bectenecin, BLP2, C1orf85, C1rb, C1rL, C230078M08Rik, C5orf21, Calpain II, CARD 8, Cbl-3, Chromozym TRY, CLN1, CPZ, CRISP-8, CTBS, cystatin 11, Desc4, DKFZp761E198, EDEM, EDEM2, EG433016, Enterokinase LC, ERAP2, FBXW2, HAI-1, HAI-2, HSBP1L1, iGb3, KIAA0776, KLK15, Klk1b4, Klk26, KLK9, KLKb2, KLKb8, LAPTM5, Legumain, LIMP I, Lyst, MARCH4, MASP-2, MFSD8, MON1A, murinoglobulin 2, NEURL1B, Ocathepsin, PACE4, Phosphoramidon, PN-1, PRDM14, Prss28, Prss29, Prss39, Prss47, RNase T2, RNF31, SERHL, SerpinA1c, SerpinA3i, SerpinA3m, SerpinB1b, Serpinb3b, SerpinB6C, SerpinB9g, stefin A1, stefin A1L1, stefin A2, stefin A3, Syntaxin 12, TAP2, Tesp2, TESSP-1, Thimet oligopeptidase, TMEM9, TMPRSS11B, TMPRSS11C, TMPRSS11E2, TMPRSS11F, TMPRSS13, TMPRSS3, TMPRSS5, TMPRSS7, TMPRSSs8, TPPII, TPSB1, tsg 101, UBC8, Ubr1, UNQ9391, WDR91, and ZFYVE26.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E-64, 5 mg | sc-201276 | 5 mg | $281.00 | |||
E-64, 25 mg | sc-201276A | 25 mg | $947.00 | |||
E-64, 250 mg | sc-201276B | 250 mg | $1574.00 |