Date published: 2025-9-5

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Dynamin I 抗体 (D5): sc-12724

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说明书
  • Dynamin I 抗体 D5 是小鼠单克隆 IgG1 κ,Dynamin I抗体, 在13篇文献中引用,规格为200 µg/ml
  • 免疫human物种的recombinant Dynamin I的氨基酸1-750
  • Dynamin I 抗体 (D5) 推荐用于 WB, IP 和 IF,检测mouse, rat, human, canine 和avian 来源的 Dynamin I
  • 抗Dynamin I抗体(D5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dynamin I (D5): sc-12724免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Dynamin I Antibody (D5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Dynamin I Antibody (D5) 打包提供(请参阅下面的订购信息)。

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関連項目

Dynamin I抗体(D5)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠、人、犬和鸟类来源的Dynamin I蛋白。抗Dynamin I抗体(D5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Dynamin I在胞吞作用、突触传递和神经发生中发挥着关键作用,因此Dynamin I对于细胞通讯和维持神经元健康至关重要。Dynamin I主要定位于中枢神经系统,参与形成包被有衣藻体的囊泡,这一过程对于神经递质和其他信号分子的内化至关重要。Dynamin I的结构包括SH3和富含脯氨酸的域,这些结构有助于Dynamin I与各种效应蛋白相互作用,而效应蛋白对于GTP酶活性和随后的内吞功能是必不可少的。值得注意的是,Dynamin I通过与amphiphysin相互作用而被招募到包被有clathrin的凹坑中,amphiphysin是一种在大脑中高度表达的蛋白,这强调了这些相互作用在调节突触囊泡回收和神经信号传导中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dynamin I 抗体 (D5) 参考文献:

  1. Cdk5 对phiphysin I 和 dynamin I 的共磷酸化调节了凝集素介导的突触囊泡的内吞作用。  |  Tomizawa, K., et al. 2003. J Cell Biol. 163: 813-24. PMID: 14623869
  2. 活力素 I 磷酸化与突触囊泡内吞的控制。  |  Smillie, KJ. and Cousin, MA. 2005. Biochem Soc Symp. 87-97. PMID: 15649133
  3. Syndapin I是磷酸化调控的动态素I在突触囊泡内吞过程中的伙伴。  |  Anggono, V., et al. 2006. Nat Neurosci. 9: 752-60. PMID: 16648848
  4. 动力蛋白I与NAP-22(一种富含突触前区域的神经元蛋白)的相互作用。  |  Ueno, S., et al. 2018. Neurosci Lett. 675: 59-63. PMID: 29604406
  5. 生长因子诱导的达纳明与信号转导蛋白的结合涉及到对不同的富含脯氨酸的达纳明序列的分选。  |  Scaife, R., et al. 1994. EMBO J. 13: 2574-82. PMID: 8013457
  6. 鉴定大鼠组织中普遍表达的同工酶达纳明 2。  |  Cook, TA., et al. 1994. Proc Natl Acad Sci U S A. 91: 644-8. PMID: 8290576
  7. 多种动态蛋白的差异表达和调控。  |  Sontag, JM., et al. 1994. J Biol Chem. 269: 4547-54. PMID: 8308025
  8. SH3结构域的结合位点可将动态蛋白靶向涂布坑。  |  Shpetner, HS., et al. 1996. J Biol Chem. 271: 13-6. PMID: 8550547
  9. 达纳明富含脯氨酸的基本区域在 Src 同源 3 结构域结合和内吞过程中的作用  |  Okamoto, PM., et al. 1997. J Biol Chem. 272: 11629-35. PMID: 9111080
  10. 虹彩蛋白的 SH3 结构域与达能蛋白的富脯氨酸结构域结合在一个单一位点上,该位点定义了一个新的 SH3 结合共识序列。  |  Grabs, D., et al. 1997. J Biol Chem. 272: 13419-25. PMID: 9148966
  11. 虹彩蛋白 SH3 结构域对受体介导的内吞作用的抑制。  |  Wigge, P., et al. 1997. Curr Biol. 7: 554-60. PMID: 9259551
  12. PH 结构域和 SH3 结合结构域在达能素功能中的作用  |  Scaife, RM. and Margolis, RL. 1997. Cell Signal. 9: 395-401. PMID: 9376220

订购信息

产品名称产品编号规格价格数量收藏夹

Dynamin I 抗体 (D5)

sc-12724
200 µg/ml
$316.00

Dynamin I (D5): m-IgG Fc BP-HRP 套装

sc-528250
200 µg Ab; 10 µg BP
$354.00

Dynamin I (D5): m-IgGκ BP-HRP 套装

sc-520580
200 µg Ab, 40 µg BP
$354.00

Dynamin I (D5): m-IgG1 BP-HRP 套装

sc-542840
200 µg Ab; 20 µg BP
$354.00

Dynamin I 抗体 (D5) AC

sc-12724 AC
500 µg/ml, 25% agarose
$416.00

Dynamin I 抗体 (D5) HRP

sc-12724 HRP
200 µg/ml
$316.00

Dynamin I 抗体 (D5) FITC

sc-12724 FITC
200 µg/ml
$330.00

Dynamin I 抗体 (D5) PE

sc-12724 PE
200 µg/ml
$343.00

Dynamin I 抗体 (D5) Alexa Fluor® 488

sc-12724 AF488
200 µg/ml
$357.00

Dynamin I 抗体 (D5) Alexa Fluor® 546

sc-12724 AF546
200 µg/ml
$357.00

Dynamin I 抗体 (D5) Alexa Fluor® 594

sc-12724 AF594
200 µg/ml
$357.00

Dynamin I 抗体 (D5) Alexa Fluor® 647

sc-12724 AF647
200 µg/ml
$357.00

Dynamin I 抗体 (D5) Alexa Fluor® 680

sc-12724 AF680
200 µg/ml
$357.00

Dynamin I 抗体 (D5) Alexa Fluor® 790

sc-12724 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with Dynamin I (D5): sc-12724 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from very well working antibodyGot clear bands with sc-12724 sc mouse monoclonal antibody in rat heart samples
Date published: 2017-03-16
Rated 5 out of 5 by from Robust signal in WB in human Dynamin I transfectedRobust signal in WB in human Dynamin I transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-06-07
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in methanol-fixed SK-N-SH cells. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Published data for WB in human 293 FT andPublished data for WB in human 293 FT and HeLa cells. -SCBT Publication Review
Date published: 2015-03-10
Rated 5 out of 5 by from Published IHC(P) data for use in monkey brainPublished IHC(P) data for use in monkey brain. -SCBT Publication Review
Date published: 2015-02-26
Rated 5 out of 5 by from GoodGood, strong band observed in WB in rat brain and mouse brain extracts and SK-N-SH whole cell lysate. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Published data for CoIP and WB in human H295RPublished data for CoIP and WB in human H295R adrenocortical cells. -SCBT Publication Review
Date published: 2015-02-10
Rated 5 out of 5 by from Worked great in WB in SHWorked great in WB in SH-SY5Y whole cell lysate and human cerebral cortex tissue extract. -SCBT QC
Date published: 2015-01-26
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Dynamin I 抗体 (D5) is rated 5.0 out of 5 by 8.
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