DUE-B Antibody (D-7): sc-515087
- DUE-B Antibody (D-7) is a mouse monoclonal IgG2a κ DUE-B antibody provided at 200 µg/ml
- raised against amino acids 1-159 mapping at the N-terminus of DUE-B of human origin
- DUE-B Antibody (D-7) is recommended for detection of DUE-B of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DUE-B Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DUE-B Antibody (D-7) for WB applications. These reagents are now offered in bundles with DUE-B Antibody (D-7) (see ordering information below).
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DUE-B Antibody (D-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects DUE-B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-DUE-B antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DUE-B, also known as DTD1 (D-tyrosyl-tRNA deacylase 1) or HARS2, consists of 209 amino acids and is primarily localized in the cytoplasm, where DUE-B plays a crucial role in cellular metabolism. DUE-B is involved in the hydrolysis of D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr), a process vital for maintaining cellular homeostasis and may act as a defense mechanism against D-tyrosine accumulation, which can be toxic at high levels. DUE-B is also implicated in the S phase of the cell cycle and is essential for DNA replication initiation, highlighting DUE-B′s importance in cell division and growth. DUE-B′s encoding gene is located on human chromosome 20, which contains over 600 genes and represents nearly 2% of the human genome, indicating DUE-B′s evolutionary significance and potential conservation of function across species, as human DUE-B shares 94% sequence identity with its mouse counterpart.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DUE-B Antibody (D-7) References:
- D-tyrosyl-tRNA(Tyr) metabolism in Saccharomyces cerevisiae. | Soutourina, J., et al. 2000. J Biol Chem. 275: 11626-30. PMID: 10766779
- Cloning and identification of a novel cDNA which may be associated with FKBP25. | Meng, X., et al. 2002. Biochem Genet. 40: 303-10. PMID: 12392168
- The c-myc DNA-unwinding element-binding protein modulates the assembly of DNA replication complexes in vitro. | Casper, JM., et al. 2005. J Biol Chem. 280: 13071-83. PMID: 15653697
- The synthesis and initial characterization of an immobilized DNA unwinding element binding (DUE-B) protein chromatographic stationary phase. | Moaddel, R., et al. 2005. J Chromatogr B Analyt Technol Biomed Life Sci. 820: 197-203. PMID: 15899373
- Differential binding of replication proteins across the human c-myc replicator. | Ghosh, M., et al. 2006. Mol Cell Biol. 26: 5270-83. PMID: 16809765
- Structure and function of the c-myc DNA-unwinding element-binding protein DUE-B. | Kemp, M., et al. 2007. J Biol Chem. 282: 10441-8. PMID: 17264083
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DUE-B Antibody (D-7) | sc-515087 | 200 µg/ml | $316.00 | |||
DUE-B Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-531284 | 200 µg Ab; 10 µg BP | $354.00 | |||
DUE-B Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-524930 | 200 µg Ab, 40 µg BP | $354.00 | |||
DUE-B Antibody (D-7): m-IgG2a BP-HRP Bundle | sc-547752 | 200 µg Ab; 10 µg BP | $354.00 | |||
DUE-B Antibody (D-7) AC | sc-515087 AC | 500 µg/ml, 25% agarose | $416.00 | |||
DUE-B Antibody (D-7) HRP | sc-515087 HRP | 200 µg/ml | $316.00 | |||
DUE-B Antibody (D-7) FITC | sc-515087 FITC | 200 µg/ml | $330.00 | |||
DUE-B Antibody (D-7) PE | sc-515087 PE | 200 µg/ml | $343.00 | |||
DUE-B Antibody (D-7) Alexa Fluor® 488 | sc-515087 AF488 | 200 µg/ml | $357.00 | |||
DUE-B Antibody (D-7) Alexa Fluor® 546 | sc-515087 AF546 | 200 µg/ml | $357.00 | |||
DUE-B Antibody (D-7) Alexa Fluor® 594 | sc-515087 AF594 | 200 µg/ml | $357.00 | |||
DUE-B Antibody (D-7) Alexa Fluor® 647 | sc-515087 AF647 | 200 µg/ml | $357.00 | |||
DUE-B Antibody (D-7) Alexa Fluor® 680 | sc-515087 AF680 | 200 µg/ml | $357.00 | |||
DUE-B Antibody (D-7) Alexa Fluor® 790 | sc-515087 AF790 | 200 µg/ml | $357.00 |