DU 145 nuclear extract: sc-24960
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; prostate carcinoma cells
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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The DU 145 nuclear extract originates from the DU 145 cell line, a human prostate cancer cell line often utilized in cancer research. This cell line was originally derived from a metastatic site of a human prostate carcinoma, making it particularly relevant for studies on cancer metastasis and progression. The nuclear extract from DU 145 cells contains a rich array of nuclear proteins, including transcription factors, DNA repair enzymes, and chromatin remodeling proteins, which are essential for understanding the regulation of gene expression and nuclear dynamics in prostate cancer cells. Researchers use the DU 145 nuclear extract to delve into the molecular mechanisms that drive prostate cancer, focusing on how changes in the nuclear environment affect cellular behavior, gene regulation, and the stability of the genome. By studying this extract, scientists can explore the interactions between nuclear proteins and DNA, investigate the pathways that lead to cellular transformation, and understand the nuclear response to various external and internal stimuli. The research using DU 145 nuclear extract helps to explain the complex biological processes involved in cancer biology, offering insights into the nuclear aspects of prostate cancer development and providing a valuable tool for fundamental cancer research, excluding direct applications in diagnosis or management.
DU 145 nuclear extract References:
- Zn2+-chelating motif-tethered short-chain fatty acids as a novel class of histone deacetylase inhibitors. | Lu, Q., et al. 2004. J Med Chem. 47: 467-74. PMID: 14711316
- Structure-based optimization of phenylbutyrate-derived histone deacetylase inhibitors. | Lu, Q., et al. 2005. J Med Chem. 48: 5530-5. PMID: 16107152
- Histone deacetylase inhibitors (HDACIs). Structure--activity relationships: history and new QSAR perspectives. | Pontiki, E. and Hadjipavlou-Litina, D. 2012. Med Res Rev. 32: 1-165. PMID: 20162725
- Next generation histone deacetylase inhibitors: the answer to the search for optimized epigenetic therapies? | Thaler, F. and Minucci, S. 2011. Expert Opin Drug Discov. 6: 393-404. PMID: 22646017
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DU 145 nuclear extract | sc-24960 | 250 µg/0.05 ml | $160.00 |