Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DTYMK CRISPR/Cas9 KO Plasmid (m): sc-423426
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DTYMK CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DTYMK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DTYMK Antibody (B-8): sc-365925
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DTYMK CRISPR/Cas9 KO Plasmid (m) | sc-423426 | 20 µg | $397.00 |
Overview
Dtymk encodes deoxythymidylate kinase (DTYMK), an essential cytosolic enzyme that phosphorylates dTMP to dTDP, sustaining dTTP pools required for DNA replication and repair. By linking de novo thymidylate synthesis with nucleotide salvage, DTYMK supports S-phase progression, genome stability, and mitochondrial and nuclear DNA maintenance through balanced dNTP homeostasis. Perturbation of dTTP availability can elevate replication stress and DNA damage signaling, connecting DTYMK function to pathways controlling cell-cycle checkpoints and chromosomal integrity. DTYMK has been implicated in proliferative and neurodevelopmental phenotypes in model systems, making Dtymk a useful node for studying nucleotide metabolism–associated disease mechanisms without implying clinical outcomes.
DTYMK CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dtymk gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dtymk together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dtymk open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DTYMK protein expression.
This CRISPR knockout system enables efficient generation of Dtymk-deficient cell models for investigation of DTYMK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dtymk exon(s) critical for DTYMK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dtymk genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DTYMK CRISPR/Cas9 KO Plasmid (m) and DTYMK CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dtymk locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DTYMK HDR Plasmid (m) and DTYMK HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dtymk homology arms to support homology-directed repair at defined Dtymk target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.