Molecular structure of DTT, CAS Number: 27565-41-9
DTT (CAS 27565-41-9)
See product citations (28)
Alternate Names:
DL-Dithiothreitol; Cleland′s Reagent; Dithiothreitol; 1,4-Dithio-DL-threitol; DL-Dithiothreitol; Sputolysin; Threo-1,4-dimercaptobutane-2,3-diol; DL-Threo-1,4-dimercapto-2,3-butanediol; 1,4-Dithio-DL-threitol; Reductacryl; (2S,3S)-1,4-dimercaptobutane-2,3-diol; threitol, 1, 4-dithio-, DL-; 2, 3-butanediol, 1, 4-dimercapto-, DL-threo-; 2, 3-butanediol, 1, 4-dimercapto-, (R*, R*)-(±)-; DL-threo-1, 4-dimercapto-2, 3-butanediol; threo-1, 4-dimercapto-2, 3-butanediol; (R*, R*)-1(±)-1, 4-dimercapto-2, 3-butanediol; DL-dithiothreitol; DL-1, 4-dithiothreitol; rac-dithiothreito
Application:
DTT is a cell permeable protective agent for Thiol groups which keeps monothiols in the reduced state and reduces disulfides
CAS Number:
27565-41-9
Purity:
>97%
Molecular Weight:
154.25
Molecular Formula:
C4H10O2S2
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
DTT (1,4-Dithiothreitol) acts as a cell permeable protective agent for Thiol groups. It keeps monothiols completely in the reduced state and reduces disulfides quantitatively. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. Since it readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. It is less pungent and is less toxic than 2-mercaptoethanol. It has been reported that a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol.
DTT (CAS 27565-41-9) References
- DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS. | CLELAND, WW. 1964. Biochemistry. 3: 480-2. PMID: 14192894
- The effect of surface attachment on ligand binding: studying the association of Mg2+, Ca2+ and Sr2+ by 1-thioglycerol and 1,4-dithiothreitol monolayers. | Burshtain, D. and Mandler, D. 2006. Phys Chem Chem Phys. 8: 158-64. PMID: 16482256
- Molecular mechanism for prevention of N-acetyl-p-benzoquinoneimine cytotoxicity by the permeable thiol drugs diethyldithiocarbamate and dithiothreitol. | Lauriault, VV. and O'Brien, PJ. 1991. Mol Pharmacol. 40: 125-34. PMID: 1649964
- A highly selective ratiometric fluorescent probe for 1,4-dithiothreitol (DTT) detection. | Zhu, B., et al. 2010. Org Biomol Chem. 8: 1650-4. PMID: 20237677
- A Colorimetric Sensor for the Highly Selective Detection of Sulfide and 1,4-Dithiothreitol Based on the In Situ Formation of Silver Nanoparticles Using Dopamine. | Zhao, L., et al. 2017. Sensors (Basel). 17: PMID: 28335506
- Enzyme activity and sulfhydryl status in rat renal cortex following mercuric chloride and dithiothreitol administration. | Klonne, DR. and Johnson, DR. 1988. Toxicol Lett. 42: 199-205. PMID: 2841777
- A new xanthene-based two-photon fluorescent probe for the imaging of 1,4-dithiothreitol (DTT) in living cells. | Wang, C., et al. 2018. Luminescence. 33: 1048-1053. PMID: 29927540
- Two-photon imaging of 1,4-dithiothreitol (DTT) by a red-emissive fluorescent probe in living cells, tissues and animals. | Zhang, N., et al. 2018. Spectrochim Acta A Mol Biomol Spectrosc. 205: 528-533. PMID: 30077141
- 1,4-Dithiothreitol treatment ameliorates hematopoietic and intestinal injury in irradiated mice: Potential application of a treatment for acute radiation syndrome. | Li, K., et al. 2019. Int Immunopharmacol. 76: 105913. PMID: 31627170
- Selective detection of nanomolar in aqueous solution based on functionalized gold nanoparticles. | Tan, F., et al. 2011. Anal Methods. 3: 343-347. PMID: 32938034
- The development of an endoplasmic reticulum-targeting fluorescent probe for the imaging of 1,4-dithiothreitol (DTT) in living cells. | Mehmood, AH., et al. 2021. Anal Methods. 13: 2204-2208. PMID: 33904541
- Inhibitor potency and assay conditions: A case study on SARS-CoV-2 main protease. | Behnam, MAM. and Klein, CD. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34475216
- Distinct Roles of Nrf1 and Nrf2 in Monitoring the Reductive Stress Response to Dithiothreitol (DTT). | Wufuer, R., et al. 2022. Antioxidants (Basel). 11: PMID: 36009254
- Cleland's reagents block lethal and hypnotic effects of pentobarbital. | Gailis, L. 1994. Eur J Pharmacol. 292: 39-42. PMID: 7867688
- Selective potentiation of histamine H1-receptor stimulated calcium responses by 1,4-dithiothreitol in DDT1MF-2 cells. | Dickenson, JM. and Hill, SJ. 1994. Biochem Pharmacol. 48: 1721-8. PMID: 7980641
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DTT, 1 g | sc-29089 | 1 g | $65.00 | |||
DTT, 5 g | sc-29089A | 5 g | $185.00 | |||
DTT, 10 g | sc-29089B | 10 g | $315.00 | |||
DTT, 100 g | sc-29089C | 100 g | $700.00 | |||
DTT, 1 kg | sc-29089D | 1 kg | $2960.00 |