Date published: 2026-4-24

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DTME (CAS 71865-37-7)

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Alternate Names:
Dithio-bis-maleimidoethane
Application:
DTME is a homobifunctional , thiol-cleavable, sulfhydryl-reactive crosslinker, 13.3 angstrom spacer arm
CAS Number:
71865-37-7
Molecular Weight:
312.37
Molecular Formula:
C12H12N2O4S2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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DTME, also known as Dithio-bis-maleimidoethane, functions as a crosslinking agent in biology and biochemistry. Its mechanism of action involves the formation of covalent bonds with thiol groups on proteins, peptides, or other cellular components. This interaction leads to the formation of stable crosslinks between these molecules, which can be utilized for various purposes in the study of cellular processes. By covalently linking thiol-containing molecules, DTME can be used to study protein-protein interactions, protein structure, and the organization of cellular components. DTME can be employed to immobilize proteins or peptides onto solid supports for various applications, such as affinity chromatography or protein microarray technology. The ability of DTME to form stable crosslinks with thiol-containing molecules makes it valuable for investigating the structure and function of cellular and molecular components in biology.


DTME (CAS 71865-37-7) References

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  2. PEG-assisted DNA solubilization in organic solvents for preparing cytosol specifically degradable PEG/DNA nanogels.  |  Mok, H. and Park, TG. 2006. Bioconjug Chem. 17: 1369-72. PMID: 17105212
  3. Mapping the cofilin binding site on yeast G-actin by chemical cross-linking.  |  Grintsevich, EE., et al. 2008. J Mol Biol. 377: 395-409. PMID: 18258262
  4. Nedd4 mediates agonist-dependent ubiquitination, lysosomal targeting, and degradation of the beta2-adrenergic receptor.  |  Shenoy, SK., et al. 2008. J Biol Chem. 283: 22166-76. PMID: 18544533
  5. Multimeric small interfering ribonucleic acid for highly efficient sequence-specific gene silencing.  |  Mok, H., et al. 2010. Nat Mater. 9: 272-8. PMID: 20098433
  6. β-arrestin1 mediates metastatic growth of breast cancer cells by facilitating HIF-1-dependent VEGF expression.  |  Shenoy, SK., et al. 2012. Oncogene. 31: 282-92. PMID: 21685944
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  8. Spin selectivity in electron transfer in photosystem I.  |  Carmeli, I., et al. 2014. Angew Chem Int Ed Engl. 53: 8953-8. PMID: 24989350
  9. Deciphering the galectin-12 protein interactome reveals a major impact of galectin-12 on glutamine anaplerosis in colon cancer cells.  |  Katzenmaier, EM., et al. 2019. Exp Cell Res. 379: 129-139. PMID: 30935948
  10. The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria.  |  Ge, DT., et al. 2019. Mol Cell. 74: 982-995.e6. PMID: 31076285
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  13. Enhanced Optoelectronics by Oriented Multilayers of Photosystem I Proteins in Dry Hybrid Bio-Solid Devices  |  Omri Heifler, Chanoch Carmeli, and Itai Carmeli*. 2018,. J. Phys. Chem. C. 122, 21,: 11550–11556.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

DTME, 5 mg

sc-214925A
5 mg
$203.00

DTME, 10 mg

sc-214925
10 mg
$338.00

DTME, 25 mg

sc-214925B
25 mg
$567.00

DTME, 50 mg

sc-214925C
50 mg
$1144.00

DTME, 100 mg

sc-214925D
100 mg
$2086.00