DsRed2 Antibody (25): sc-101526

DsRed2 Antibody (25) is a mouse monoclonal IgG1 kappa light chain antibody that detects DsRed2 protein of non-species reactive by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-DsRed2 antibody (25) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DsRed2 protein, a derivative of the Discosoma sp. red fluorescent protein, serves as a visual reporter in molecular biology due to exceptional brightness and photostability, crucial for real-time imaging of cellular processes. A beta-barrel configuration allows for efficient chromophore formation, enabling DsRed2 protein to emit bright red fluorescence upon excitation. This structural feature enhances signal-to-noise ratio in fluorescence applications, making DsRed2 protein an ideal choice for tagging proteins in live-cell imaging and other experimental setups. DsRed2 protein′s versatility as a fusion tag facilitates the study of protein localization, interactions, and dynamics within various cellular contexts, providing valuable insights into biological functions and mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DsRed2 Antibody (25) References:

1. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.  |  Baird, GS., et al. 2000. Proc Natl Acad Sci U S A. 97: 11984-9. PMID: 11050229
2. The structural basis for red fluorescence in the tetrameric GFP homolog DsRed.  |  Wall, MA., et al. 2000. Nat Struct Biol. 7: 1133-8. PMID: 11101896
3. Red fluorescent protein (DsRed) as a reporter in Saccharomyces cerevisiae.  |  Rodrigues, F., et al. 2001. J Bacteriol. 183: 3791-4. PMID: 11371544
4. Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP.  |  Zapata-Hommer, O. and Griesbeck, O. 2003. BMC Biotechnol. 3: 5. PMID: 12769828
5. Red fluorescent protein (DsRed2) as a novel reporter in Fusarium oxysporum f. sp. lycopersici.  |  Nahalkova, J. and Fatehi, J. 2003. FEMS Microbiol Lett. 225: 305-9. PMID: 12951257
6. Establishment of Alb-DsRed2 transgenic rat for liver regeneration research.  |  Sato, Y., et al. 2003. Biochem Biophys Res Commun. 311: 478-81. PMID: 14592439
7. Simultaneous detection of DsRed2-tagged and EGFP-tagged human beta-interferons in the same single cells.  |  Maruyama, M., et al. 2004. J Cell Biochem. 93: 497-502. PMID: 15372621
8. Enhancer trapping with a red fluorescent protein reporter in Drosophila.  |  Akimoto, A., et al. 2005. Dev Dyn. 233: 993-7. PMID: 15906373
9. Crystallographic structures of Discosoma red fluorescent protein with immature and mature chromophores: linking peptide bond trans-cis isomerization and acylimine formation in chromophore maturation.  |  Tubbs, JL., et al. 2005. Biochemistry. 44: 9833-40. PMID: 16026155
10. A red fluorescent protein, DsRed2, as a visual reporter for transient expression and stable transformation in soybean.  |  Nishizawa, K., et al. 2006. Plant Cell Rep. 25: 1355-61. PMID: 16841215
11. Characterization of dsRed2-positive cells in the doublecortin-dsRed2 transgenic adult rat retina.  |  Trost, A., et al. 2014. Histochem Cell Biol. 142: 601-17. PMID: 25138677
12. Red fluorescence protein (DsRed2) promotes the screening efficiency in peanut genetic transformation.  |  Huai, D., et al. 2023. Front Plant Sci. 14: 1123644. PMID: 36938000

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DsRed2 Antibody (25) has 28 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DsRed2 Antibody (25) Specifications

Alternate Product Name: DsRed2 fluorescent

Product Clone Name: 25

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-245

Epitope Target: DsRed2

Epitope Species: Discosoma

Product Reactivity/Detection: DsRed2

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 28 citations in various scientific publications

Available Conjugates/Forms for DsRed2 Antibody (25): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin