Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DSPP Double Nickase Plasmid (h): sc-401195-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DSPP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DSPP Double Nickase Plasmid (h) and DSPP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DSPP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DSPP Antibody (LFMb-21): sc-73632
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DSPP Double Nickase Plasmid (h) | sc-401195-NIC | 20 µg | $410.00 | |||
DSPP Double Nickase Plasmid (h2) | sc-401195-NIC-2 | 20 µg | $410.00 |
Dentin sialophosphoprotein (DSPP) is a secreted extracellular matrix precursor that is proteolytically processed into dentin sialoprotein and dentin phosphoprotein, key components that regulate mineral nucleation, crystal growth, and matrix organization in mineralized tissues. In odontoblasts, DSPP contributes to dentinogenesis by coordinating collagen-rich matrix maturation and hydroxyapatite deposition, influencing calcium/phosphate handling and extracellular matrix remodeling. Altered DSPP expression or sequence disrupts dentin mineralization dynamics and is linked to inherited dentin defects, making it a useful locus for studying genotype–phenotype relationships in craniofacial biology. DSPP also serves as a marker for odontogenic differentiation in stem cell and developmental models where extracellular matrix cues shape tissue architecture.
DSPP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DSPP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DSPP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DSPP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DSPP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.