Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Drebrin Double Nickase Plasmid (h): sc-403241-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Drebrin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Drebrin Double Nickase Plasmid (h) and Drebrin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DBN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Drebrin Antibody (C-8): sc-374269
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Drebrin Double Nickase Plasmid (h) | sc-403241-NIC | 20 µg | $410.00 | |||
Drebrin Double Nickase Plasmid (h2) | sc-403241-NIC-2 | 20 µg | $410.00 |
DBN1 encodes drebrin, an actin-binding protein enriched in neurons that regulates cytoskeletal remodeling, growth cone dynamics, and dendritic spine morphology. Drebrin modulates actin filament organization and couples synaptic signaling to structural plasticity through pathways governing cell adhesion, neurite outgrowth, and postsynaptic density assembly. Altered DBN1 expression and drebrin localization have been linked to changes in synaptic stability and circuit function, with reported associations to neurodevelopmental and neurodegenerative phenotypes. In non-neuronal contexts, drebrin also contributes to actin-dependent processes such as membrane protrusion formation and cell motility, supporting broad study of cytoskeletal regulation.
Drebrin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DBN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DBN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DBN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DBN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.