Date published: 2025-10-11

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DREAM Antibody (A-9): sc-166916

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Datasheets
  • DREAM Antibody (A-9) is a mouse monoclonal IgG2b κ DREAM antibody, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 1-214 representing full length DREAM of human origin
  • DREAM Antibody (A-9) is recommended for detection of DREAM of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-DREAM Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for DREAM Antibody (A-9) for WB applications. These reagents are now offered in bundles with DREAM Antibody (A-9) (see ordering information below).

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DREAM Antibody (A-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects DREAM protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-DREAM antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DREAM protein, known as DRE-antagonist modulator, functions as a calcium-regulated transcriptional repressor that specifically binds to downstream regulatory elements (DRE), which are crucial for silencing basal transcription. This regulatory sequence is located in the promoter region of the gene encoding human prodynorphin, an opioid peptide that plays a significant role in memory acquisition and pain modulation. DREAM′s ability to form functional homotetramers is essential for DRE interaction, and this binding is highly sensitive to calcium levels; an increase in calcium concentration inhibits DREAM′s binding capacity, thereby blocking repressive activity. DREAM transcripts are predominantly found in the brain, thymus, and thyroid gland, where DREAM is expressed as a nuclear protein. DREAM has been shown to inhibit the transcription of other genes containing DRE-like motifs, including the gene for the AP-1 transcription factor c-Fos, indicating a broader influence on various cellular genes and pathways.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

DREAM Antibody (A-9) References:

  1. Cell signalling. DREAM on without calcium.  |  Mandel, G. and Goodman, RH. 1999. Nature. 398: 29-30. PMID: 10078526
  2. DREAM is a Ca2+-regulated transcriptional repressor.  |  Carrión, AM., et al. 1999. Nature. 398: 80-4. PMID: 10078534
  3. Ca(2+)-dependent prodynorphin transcriptional derepression in neuroblastoma cells is exerted through DREAM protein activity in a kinase-independent manner.  |  Campos, D., et al. 2003. Mol Cell Neurosci. 22: 135-45. PMID: 12676525
  4. Transcriptional repressor DREAM interacts with thyroid transcription factor-1 and regulates thyroglobulin gene expression.  |  Rivas, M., et al. 2004. J Biol Chem. 279: 33114-22. PMID: 15181011
  5. Transcriptional repressor DREAM regulates T-lymphocyte proliferation and cytokine gene expression.  |  Savignac, M., et al. 2005. EMBO J. 24: 3555-64. PMID: 16177826
  6. DREAM (downstream regulatory element antagonist modulator) contributes to synaptic depression and contextual fear memory.  |  Wu, LJ., et al. 2010. Mol Brain. 3: 3. PMID: 20205763
  7. Role of ion flux in the control of c-fos expression.  |  Morgan, JI. and Curran, T. Nature. 322: 552-5. PMID: 2426600
  8. Hydrogen-Deuterium Exchange Mass Spectrometry Reveals Calcium Binding Properties and Allosteric Regulation of Downstream Regulatory Element Antagonist Modulator (DREAM).  |  Zhang, J., et al. 2017. Biochemistry. 56: 3523-3530. PMID: 28627884
  9. Pb2+ Binds to Downstream Regulatory Element Antagonist Modulator (DREAM) and Modulates Its Interactions with Binding Partners: A Link between Neuronal Calcium Sensors and Pb2+ Neurotoxicity.  |  Azam, S. and Miksovska, J. 2019. ACS Chem Neurosci. 10: 1263-1272. PMID: 30399317
  10. The opioid peptide dynorphin mediates heterosynaptic depression of hippocampal mossy fibre synapses and modulates long-term potentiation.  |  Weisskopf, MG., et al. 1993. Nature. 365: 188. PMID: 8103916
  11. Differential messenger RNA expression of prodynorphin and proenkephalin in the human brain.  |  Hurd, YL. 1996. Neuroscience. 72: 767-83. PMID: 9157322
  12. Protein kinase A-dependent derepression of the human prodynorphin gene via differential binding to an intragenic silencer element.  |  Carrión, AM., et al. 1998. Mol Cell Biol. 18: 6921-9. PMID: 9819380

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

DREAM Antibody (A-9)

sc-166916
200 µg/ml
$316.00

DREAM Antibody (A-9): m-IgG Fc BP-HRP Bundle

sc-525774
200 µg Ab; 10 µg BP
$354.00

DREAM Antibody (A-9): m-IgG2b BP-HRP Bundle

sc-549633
200 µg Ab; 10 µg BP
$354.00

DREAM Antibody (A-9) AC

sc-166916 AC
500 µg/ml, 25% agarose
$416.00

DREAM Antibody (A-9) HRP

sc-166916 HRP
200 µg/ml
$316.00

DREAM Antibody (A-9) FITC

sc-166916 FITC
200 µg/ml
$330.00

DREAM Antibody (A-9) PE

sc-166916 PE
200 µg/ml
$343.00

DREAM Antibody (A-9) Alexa Fluor® 488

sc-166916 AF488
200 µg/ml
$357.00

DREAM Antibody (A-9) Alexa Fluor® 546

sc-166916 AF546
200 µg/ml
$357.00

DREAM Antibody (A-9) Alexa Fluor® 594

sc-166916 AF594
200 µg/ml
$357.00

DREAM Antibody (A-9) Alexa Fluor® 647

sc-166916 AF647
200 µg/ml
$357.00

DREAM Antibody (A-9) Alexa Fluor® 680

sc-166916 AF680
200 µg/ml
$357.00

DREAM Antibody (A-9) Alexa Fluor® 790

sc-166916 AF790
200 µg/ml
$357.00

I received the free sample of DREAM SC-166916 antibody and I got five different spots in Western Blot assay (using astrocytes samples from mice): 32 kDa (should be DREAM), 40 kDa, 48 kDa, 70 kDa and 80 kDa approximately. What could be those spots?

Asked by: Miroslava
Thank you for your product review. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

What is the recommended fixation method for immunofluorescence staining with DREAM (A-9): sc-166916 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24

we bought this product a few weeks ago. I'd like know that why this antibody detect both size of dream about 34kDa and 20kDa as ur datasheet mention. please let me know the reason that 20kDa size of dream

Asked by: rosalind
Thank you for your question. On our product datasheet for this mouse monoclonal antibody, DREAM (A-9): sc-166916, we list the predicted molecular weight for the DREAM protein as 32 kDa. It appears that your question is referring to the Western blot data published on the product datasheet. This data is not representative of detection of endogenous DREAM protein. The antibody was used to detect DREAM protein in a transfected whole cell lysate. The full-length DREAM protein was observed in the transfected lysate around 32 kDa. The lower molecular weight band has not been characterized, but it is presumed to be an incomplete translation product of the transfected cDNA.
Answered by: Technical Support
Date published: 2016-10-03
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Rated 1 out of 5 by from This antibody did not work at all.We tested this antibody in lysates of several cells expressing DREAM, but it did not work.
Date published: 2020-03-11
Rated 5 out of 5 by from Great monoclonal antibodyNice strong band observed with DREAM Antibody (A-9). IF stain was robust and specific
Date published: 2017-02-09
Rated 5 out of 5 by from All is okWe can see that DREAM has been shown to inhibit the transcription of proteins containing DRE-like motifs, including genes encoding the c-Fos protein for the AP-1 transcription factor, suggesting that DREAM may affect the genes of many cells.
Date published: 2017-01-24
Rated 5 out of 5 by from Good WB data with Mouse liverI bought this new antibody recently,got the good results,i used it with 1:1000,and the new antibody is very good for past and future
Date published: 2017-01-07
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-19
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DREAM Antibody (A-9) is rated 4.3 out of 5 by 6.
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