Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DR4 Double Nickase Plasmid (h): sc-400747-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DR4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DR4 Double Nickase Plasmid (h) and DR4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TNFRSF10A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DR4 Antibody (B-9): sc-8411
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DR4 Double Nickase Plasmid (h) | sc-400747-NIC | 20 µg | $410.00 | |||
DR4 Double Nickase Plasmid (h2) | sc-400747-NIC-2 | 20 µg | $410.00 |
TNFRSF10A encodes death receptor 4 (DR4), a cell-surface member of the TNF receptor superfamily that binds TRAIL and initiates extrinsic apoptosis. Upon ligand engagement, DR4 promotes death-inducing signaling complex assembly and caspase-8 activation, with downstream crosstalk to mitochondrial amplification and NF-κB-associated stress responses. DR4 signaling is integrated with pathways that regulate cell fate decisions, including apoptosis, inflammatory signaling, and immune surveillance. Altered expression or functional attenuation of TNFRSF10A has been studied in contexts of tumor biology and resistance to apoptosis-associated cues, as well as in broader dysregulation of death receptor signaling networks.
DR4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TNFRSF10A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TNFRSF10A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TNFRSF10A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TNFRSF10A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.