Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DPYD Double Nickase Plasmid (h): sc-403396-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DPYD Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DPYD Double Nickase Plasmid (h) and DPYD Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DPYD. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DPYD Antibody (A-5): sc-376712
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DPYD Double Nickase Plasmid (h) | sc-403396-NIC | 20 µg | $410.00 | |||
DPYD Double Nickase Plasmid (h2) | sc-403396-NIC-2 | 20 µg | $410.00 |
DPYD encodes dihydropyrimidine dehydrogenase, the rate-limiting enzyme in pyrimidine catabolism that converts uracil and thymine to dihydrouracil and dihydrothymine, linking nucleotide turnover to cellular nitrogen and carbon metabolism. As a major NADPH-dependent oxidoreductase, DPYD activity influences nucleotide pool homeostasis and can intersect with redox balance and mitochondrial bioenergetic demands in proliferating cells. Genetic variation or altered expression of DPYD is associated with inborn errors of pyrimidine metabolism and has been studied in the context of inter-individual differences in fluoropyrimidine drug metabolism and toxicity risk. DPYD also serves as a useful node for investigating metabolic remodeling, stress responses to nucleotide imbalance, and pathway crosstalk affecting RNA/DNA synthesis.
DPYD Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DPYD locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DPYD. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DPYD function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DPYD-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.