Dpl CRISPR/Cas9 KO Plasmid (h): sc-403482
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dpl CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dpl genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dpl CRISPR/Cas9 KO Plasmid (h) | sc-403482 | 20 µg | $397.00 |
Overview
PRND encodes the human Doppel (Dpl) protein, a glycosylphosphatidylinositol-anchored membrane glycoprotein in the prion protein family that localizes to the cell surface and secretory pathway compartments. Dpl has been linked to neuronal and reproductive biology, with reported roles in cell–cell interactions, oxidative stress responses, and regulation of survival signaling, intersecting with pathways that influence mitochondrial function and redox homeostasis. Aberrant PRND/Dpl expression has been observed in several malignancies and has been explored as a marker of altered differentiation and invasiveness, supporting investigation of its contribution to tumor cell phenotypes. In the nervous system context, prion-family proteins are relevant to protein quality control and neurodegeneration-associated processes, making PRND a useful node for mechanistic studies of membrane-associated signaling and stress adaptation.
Dpl CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRND gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRND together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRND open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dpl protein expression.
This CRISPR knockout system enables efficient generation of PRND-deficient cell models for investigation of Dpl signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRND exon(s) critical for Dpl function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRND genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dpl CRISPR/Cas9 KO Plasmid (h) and Dpl CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRND locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dpl HDR Plasmid (h) and Dpl HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRND homology arms to support homology-directed repair at defined PRND target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.