DPF1 Double Nickase Plasmid (h): sc-409539-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DPF1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DPF1 Double Nickase Plasmid (h) and DPF1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DPF1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DPF1 Double Nickase Plasmid (h) | sc-409539-NIC | 20 µg | $410.00 | |||
DPF1 Double Nickase Plasmid (h2) | sc-409539-NIC-2 | 20 µg | $410.00 |
DPF1 (double PHD fingers 1; also known as BAF45B) encodes a chromatin-associated subunit of SWI/SNF (BAF) remodeling complexes that interprets histone marks via its PHD finger domains to modulate nucleosome accessibility. Through regulation of enhancer and promoter activity, DPF1 contributes to transcriptional programs linked to neural development, lineage specification, and stimulus-responsive gene expression. Perturbation of BAF complex composition and chromatin remodeling is broadly implicated in dysregulated differentiation and oncogenic transcriptional states, making DPF1 a useful target for studying epigenetic control of cell fate and context-dependent transcription. In human cells, DPF1-focused experiments support mechanistic interrogation of chromatin remodeling–coupled pathways that shape gene expression networks relevant to development and disease-associated transcriptional rewiring.
DPF1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DPF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DPF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DPF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DPF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.