Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dok-1 Double Nickase Plasmid (h): sc-401398-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dok-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dok-1 Double Nickase Plasmid (h) and Dok-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DOK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dok-1 Antibody (A-3): sc-6929
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dok-1 Double Nickase Plasmid (h) | sc-401398-NIC | 20 µg | $410.00 | |||
Dok-1 Double Nickase Plasmid (h2) | sc-401398-NIC-2 | 20 µg | $410.00 |
Human DOK1 encodes Dok-1, a cytoplasmic docking/adaptor protein that becomes tyrosine-phosphorylated downstream of receptor and non-receptor tyrosine kinases and assembles inhibitory signaling complexes. Through interactions with SH2/SH3-containing partners, Dok-1 modulates pathways including Ras/MAPK and PI3K/AKT to constrain mitogenic and survival signaling, shaping adhesion, migration, and hematopoietic cell responses. In immune and myeloid lineages, Dok-1 contributes to negative feedback control of cytokine and growth factor signaling and can influence cell cycle progression and differentiation programs. Dysregulated DOK1 expression or signaling has been associated with altered tyrosine kinase network activity in cancer and with perturbations in immune signaling contexts, supporting its use in pathway and mechanism studies.
Dok-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DOK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DOK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DOK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DOK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.