Dodecyltrimethylammonium Chloride (CAS 112-00-5)

Dodecyltrimethylammonium chloride (DTAC) is a quaternary ammonium compound extensively studied for its surfactant properties and various research applications. Its mechanism of action primarily involves disrupting the structure and function of biological membranes. DTAC′s hydrophobic tail interacts with lipid bilayers, while its positively charged headgroup interacts with negatively charged components on cell membranes, leading to membrane destabilization. This disrupts membrane integrity and permeability, causing leakage of cellular contents and ultimately cell death. In research, DTAC has been widely employed as a surfactant in molecular biology techniques such as DNA extraction, purification, and manipulation. Its ability to solubilize and denature proteins makes it valuable in protein extraction and membrane protein solubilization. Additionally, DTAC is utilized in various analytical methods, including spectrophotometry and chromatography, for its ability to enhance solubility and facilitate separation of analytes. Furthermore, DTAC′s surfactant properties have found applications in nanoparticle synthesis, drug delivery systems, and colloidal stabilization. Overall, DTAC′s mechanism of action in disrupting biological membranes and its versatile applications in research make it a valuable tool in various scientific disciplines, ranging from molecular biology to nanotechnology.