DOCK 4 Antibody (R6Y): sc-100718

DOCK 4 Antibody (R6Y) is a mouse monoclonal IgG2b kappa light chain antibody that detects DOCK 4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DOCK 4 (R6Y) antibody is available as the non-conjugated form. DOCK 4 protein, also known as Dedicator of cytokinesis protein 4, is a cytoplasmic peripheral membrane protein that plays a crucial role in cellular processes by acting as a guanine nucleotide exchange factor (GEF) for the small GTPase Rap 1. This activation is vital for the regulation of adherens junctions, which are essential for maintaining cell-cell adhesion and tissue integrity. DOCK 4 is expressed ubiquitously, with the highest levels found in the prostate, ovary, and skeletal muscle, highlighting its importance in various physiological contexts. Structurally, DOCK 4 features an N-terminal SH3 domain, a C-terminal proline-rich region, and two internal DOCK homology regions, DHR1 and DHR2, which are critical for its function and interaction with other proteins. Notably, defects in the gene encoding DOCK 4 can lead to the inactivation of Rap 1, contributing to the development of several cancers, including ovarian, prostate, glioma, and colorectal carcinomas. Additionally, alternative splicing of the DOCK 4 gene results in the expression of four isoforms, further diversifying its functional roles in cellular signaling and cancer biology.

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DOCK 4 Antibody (R6Y) References:

1. Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity.  |  Côté, JF. and Vuori, K. 2002. J Cell Sci. 115: 4901-13. PMID: 12432077
2. DOCK4, a GTPase activator, is disrupted during tumorigenesis.  |  Yajnik, V., et al. 2003. Cell. 112: 673-84. PMID: 12628187
3. An isoform of GTPase regulator DOCK4 localizes to the stereocilia in the inner ear and binds to harmonin (USH1C).  |  Yan, D., et al. 2006. J Mol Biol. 357: 755-64. PMID: 16464467
4. Dock4 is regulated by RhoG and promotes Rac-dependent cell migration.  |  Hiramoto, K., et al. 2006. Exp Cell Res. 312: 4205-16. PMID: 17027967
5. Specific recognition of Rac2 and Cdc42 by DOCK2 and DOCK9 guanine nucleotide exchange factors.  |  Kwofie, MA. and Skowronski, J. 2008. J Biol Chem. 283: 3088-3096. PMID: 18056264

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DOCK 4 Antibody (R6Y) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-DOCK 4 Antibody (R6Y) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DOCK 4 Antibody (R6Y) Specifications

Alternate Product Name: DOCK 4

Product Clone Name: R6Y

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1867-1967

Epitope Target: DOCK 4

Epitope Species: human

Product Reactivity/Detection: DOCK 4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG_2b kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for DOCK 4 Antibody (R6Y): N/A