DOCK 11 Double Nickase Plasmid (h): sc-406204-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DOCK 11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DOCK 11 Double Nickase Plasmid (h) and DOCK 11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DOCK11. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DOCK 11 Double Nickase Plasmid (h) | sc-406204-NIC | 20 µg | $410.00 | |||
DOCK 11 Double Nickase Plasmid (h2) | sc-406204-NIC-2 | 20 µg | $410.00 |
DOCK11 encodes a dedicator of cytokinesis (DOCK) family guanine nucleotide exchange factor that preferentially activates Rac1 to coordinate actin cytoskeleton remodeling, cell polarity, and membrane ruffling. In immune and hematopoietic cells, DOCK11-dependent Rac signaling supports adhesion dynamics, migration, and immunological synapse–associated trafficking, linking it to Rho GTPase regulatory networks and downstream MAPK and PI3K pathway crosstalk. Perturbation of DOCK11 activity can alter cytoskeletal organization and signaling amplitude in lymphocyte function and other contexts where Rac-controlled motility and morphology are central. These roles make DOCK11 a useful target for studying mechanisms of cell movement, immune cell communication, and signaling nodes that contribute to disease-associated dysregulation of cytoskeletal pathways.
DOCK 11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DOCK11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DOCK11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DOCK11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DOCK11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.