DNA pol γ2 CRISPR/Cas9 KO Plasmid (h): sc-407382
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DNA pol γ2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DNA pol γ2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNA pol γ2 CRISPR/Cas9 KO Plasmid (h) | sc-407382 | 20 µg | $397.00 |
Overview
POLG2 encodes DNA polymerase gamma subunit 2, an accessory factor that binds the catalytic POLG subunit to enhance processivity and support high-fidelity mitochondrial DNA (mtDNA) replication and repair. This protein functions within mitochondrial nucleoid biology and is critical for maintaining mtDNA copy number, genome integrity, and oxidative phosphorylation capacity. Disruption of POLG2-dependent replication dynamics can contribute to mtDNA depletion or accumulation of deletions, linking mitochondrial genome instability to neuromuscular and multisystem mitochondrial disease phenotypes. POLG2 is therefore widely studied in pathways governing mitochondrial biogenesis, replication stress responses, and metabolism-driven signaling.
DNA pol γ2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the POLG2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the POLG2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the POLG2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DNA pol γ2 protein expression.
This CRISPR knockout system enables efficient generation of POLG2-deficient cell models for investigation of DNA pol γ2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting POLG2 exon(s) critical for DNA pol γ2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple POLG2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DNA pol γ2 CRISPR/Cas9 KO Plasmid (h) and DNA pol γ2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the POLG2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DNA pol γ2 HDR Plasmid (h) and DNA pol γ2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by POLG2 homology arms to support homology-directed repair at defined POLG2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.