DNA pol δ 4 Double Nickase Plasmid (h): sc-408268-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DNA pol δ 4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DNA pol δ 4 Double Nickase Plasmid (h) and DNA pol δ 4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting POLD4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNA pol δ 4 Double Nickase Plasmid (h) | sc-408268-NIC | 20 µg | $410.00 |
POLD4 encodes DNA polymerase delta subunit 4, an accessory component of the DNA polymerase δ holoenzyme that supports high-fidelity chromosomal DNA replication and lagging-strand synthesis. Through interactions within the replisome, polymerase δ coordinates with PCNA-dependent processivity, Okazaki fragment maturation, and DNA repair synthesis during base excision repair and mismatch repair. Proper POLD4 function contributes to genome stability, replication stress tolerance, and accurate cell-cycle progression through S phase. Dysregulation of polymerase δ subunits and replication-associated pathways is frequently examined in studies of mutagenesis, chromosomal instability, and cancer-associated DNA damage response phenotypes.
DNA pol δ 4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the POLD4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within POLD4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt POLD4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of POLD4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.