Dmp1 CRISPR/Cas9 KO Plasmid (h): sc-402941
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dmp1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Dmp1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dmp1 Antibody (DMTF5I250): sc-81249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dmp1 CRISPR/Cas9 KO Plasmid (h) | sc-402941 | 20 µg | $397.00 |
Overview
Human DMTF1 encodes Dmp1, a Myb-like transcription factor that regulates cell-cycle progression by modulating the ARF–MDM2–p53 axis and intersecting with RB/E2F-dependent control of G1/S transcriptional programs. Dmp1 binds promoter elements in genes governing proliferation and checkpoint responses, thereby influencing cellular senescence, stress signaling, and oncogene-induced growth control. Altered DMTF1 activity or dosage has been linked to dysregulated proliferation and tumor suppressor network imbalance, making it a useful node for studying transcriptional control of cell fate decisions in cancer-relevant contexts.
Dmp1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DMTF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DMTF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DMTF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Dmp1 protein expression.
This CRISPR knockout system enables efficient generation of DMTF1-deficient cell models for investigation of Dmp1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DMTF1 exon(s) critical for Dmp1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DMTF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Dmp1 CRISPR/Cas9 KO Plasmid (h) and Dmp1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DMTF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Dmp1 HDR Plasmid (h) and Dmp1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DMTF1 homology arms to support homology-directed repair at defined DMTF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.