DLST Double Nickase Plasmid (h): sc-409604-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DLST Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DLST Double Nickase Plasmid (h) and DLST Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DLST. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DLST Double Nickase Plasmid (h) | sc-409604-NIC | 20 µg | $410.00 | |||
DLST Double Nickase Plasmid (h2) | sc-409604-NIC-2 | 20 µg | $410.00 |
DLST encodes dihydrolipoamide S-succinyltransferase (E2) of the mitochondrial 2-oxoglutarate dehydrogenase complex, a core enzyme of the tricarboxylic acid (TCA) cycle that converts 2-oxoglutarate to succinyl-CoA. By catalyzing succinyl group transfer to CoA and coordinating lipoyl-dependent substrate channeling, DLST supports oxidative metabolism, NADH production, and mitochondrial redox homeostasis. DLST activity interfaces with anaplerosis/cataplerosis and regulates the availability of TCA intermediates used for biosynthesis, including succinyl-CoA for heme and protein succinylation. Altered DLST function has been associated with mitochondrial metabolic dysfunction and has been implicated in contexts where mitochondrial respiration, oxidative stress responses, and proliferative metabolism are perturbed.
DLST Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DLST locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DLST. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DLST function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DLST-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.