DISP2 Double Nickase Plasmid (m): sc-431923-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DISP2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DISP2 Double Nickase Plasmid (m) and DISP2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Disp2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DISP2 Double Nickase Plasmid (m) | sc-431923-NIC | 20 µg | $410.00 | |||
DISP2 Double Nickase Plasmid (m2) | sc-431923-NIC-2 | 20 µg | $410.00 |
Disp2 encodes the mouse Dispatched homolog 2 (DISP2), a multipass membrane protein related to the Dispatched family implicated in the trafficking and extracellular release of lipid-modified Hedgehog ligands. By influencing ligand availability, DISP2 is positioned to modulate Hedgehog pathway signal propagation that governs embryonic patterning, tissue morphogenesis, and stem/progenitor cell behavior. Although DISP2 is less characterized than DISP1, dysregulated Hedgehog signaling is broadly linked to developmental abnormalities and oncogenic programs, making Disp2 a relevant node for dissecting pathway regulation. Studying DISP2 can clarify how membrane transport processes shape morphogen gradients and downstream transcriptional outputs in vivo and in cell-based models.
DISP2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Disp2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Disp2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Disp2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Disp2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.