Molecular structure of Diphtheria Toxin, CRM Mutant, CAS Number: 92092-36-9
Diphtheria Toxin, CRM Mutant (CAS 92092-36-9)
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Alternate Names:
[Glu52]-Diphtheria toxin from Corynebacterium diphtheriae; CRM 197
Application:
Diphtheria Toxin, CRM Mutant is an agent that does not have NAD:EF2 ADP-ribosyltransferase activity
CAS Number:
92092-36-9
Molecular Weight:
63000
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Diptheria Toxin, CRM Mutant, also known as CRM197, does not have NAD:EF2 ADP-ribosyltransferase activity due to an amino acid substitution of glycine to glutamic acid, that results in the alteration of the NAD+ binding site, in fragment A. The non-toxic diptheria toxin, Diptheria Toxin, CRM Mutant, can inhibit bladder smooth muscle cell proliferation through its interaction with HB-EGF. Research shows that Diptheria Toxin, CRM Mutant does not catalyze ADP ribosylation of elongation factor-2 (EF-2), and failed to induce apoptosis in Vero cells in the presence of brefeldin and okadaic acid (sc-3513). Both the mutant Diptheria Toxin, CRM Mutant and the wild type diptheria toxin are alike immunologically.
Diphtheria Toxin, CRM Mutant (CAS 92092-36-9) References
- A nontoxic diphtheria toxin analogue inhibits neonatal bladder smooth muscle cell proliferation. | Kaefer, M., et al. 2000. J Urol. 163: 580-4. PMID: 10647689
- Enhancement of diphtheria toxin-induced apoptosis in Vero cells by combination treatment with brefeldin A and okadaic acid. | Kusano, I., et al. 2001. Cell Struct Funct. 26: 279-88. PMID: 11831360
- Neuropeptide-induced transactivation of a neuronal epidermal growth factor receptor is mediated by metalloprotease-dependent formation of heparin-binding epidermal growth factor. | Shah, BH., et al. 2004. J Biol Chem. 279: 414-20. PMID: 14573593
- Extracellular cysteine/cystine redox regulates the p44/p42 MAPK pathway by metalloproteinase-dependent epidermal growth factor receptor signaling. | Nkabyo, YS., et al. 2005. Am J Physiol Gastrointest Liver Physiol. 289: G70-8. PMID: 15746213
- Multiplexed measurement of serum antibodies using an array biosensor. | Moreno-Bondi, MC., et al. 2006. Biosens Bioelectron. 21: 1880-6. PMID: 16434176
- Regulation of lysophosphatidic acid-induced epidermal growth factor receptor transactivation and interleukin-8 secretion in human bronchial epithelial cells by protein kinase Cdelta, Lyn kinase, and matrix metalloproteinases. | Zhao, Y., et al. 2006. J Biol Chem. 281: 19501-11. PMID: 16687414
- Lysophosphatidic acid decreases epidermal growth factor receptor binding in airway epithelial cells. | Kassel, KM., et al. 2007. J Pharmacol Exp Ther. 323: 109-18. PMID: 17640953
- Murine red blood cells as efficient carriers of three bacterial antigens for the production of specific and neutralizing antibodies. | Polvani, C., et al. 1991. Biotechnol Appl Biochem. 14: 347-56. PMID: 1777119
- Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. | Panigone, S., et al. 2008. Mol Endocrinol. 22: 924-36. PMID: 18187604
- Synthetic peptide representing a T-cell epitope of CRM197 substitutes as carrier molecule in a Haemophilus influenzae type B (Hib) conjugate vaccine. | Bixler, GS., et al. 1989. Adv Exp Med Biol. 251: 175-80. PMID: 2481959
- Evaluation of the non-toxic mutant of the diphtheria toxin K51E/E148K as carrier protein for meningococcal vaccines. | Pecetta, S., et al. 2016. Vaccine. 34: 1405-11. PMID: 26845738
- O-Acetylation is essential for functional antibody generation against Staphylococcus aureus capsular polysaccharide. | Scully, IL., et al. 2018. Hum Vaccin Immunother. 14: 81-84. PMID: 29182428
- Soluble Diphtheria Toxin Variant, CRM 197 was Obtained in Escherichia coli at High Productivity Using SUMO Fusion and an Adjusted Expression Strategy. | Tarahomjoo, S., et al. 2022. Protein Pept Lett. 29: 350-359. PMID: 35139782
- The amino-acid sequence of two non-toxic mutants of diphtheria toxin: CRM45 and CRM197. | Giannini, G., et al. 1984. Nucleic Acids Res. 12: 4063-9. PMID: 6427753
- Differences in the immunogenicity of native and formalinized cross reacting material (CRM197) of diphtheria toxin in mice and guinea pigs and their implications on the development and control of diphtheria vaccine based on CRMs. | Gupta, RK., et al. 1997. Vaccine. 15: 1341-3. PMID: 9302741
- Unique chemical reactivity of His-21 of CRM-197, a mutated diphtheria toxin. | Chang, JY., et al. 1998. FEBS Lett. 427: 362-6. PMID: 9637258
Inhibitor of:
C12orf65_2810006K23Rik, EF-1β, eRF3a, HARS2, HB-EGF, MRP-L20, MRP-L47, NACA1, OR4N5, QM, QRSL1, Ribosomal Protein L22, Ribosomal Protein L36aL, Ribosomal Protein S16, Ribosome (RPL; 40S; 60S), RPS4Y1, RPS4YX, and TRPV6.Activator of:
Ribosomal Protein L22, Ribosomal Protein L23, and Ribosomal Protein L3L.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Diphtheria Toxin, CRM Mutant, 0.25 mg | sc-203924 | 0.25 mg | $652.00 |