Diphtheria Toxin Antibody (3B6): sc-51868

Diphtheria Toxin Antibody (3B6) is a mouse monoclonal IgG1 antibody that detects Diphtheria Toxin protein of Corynebacterium diphtheriae origin by enzyme-linked immunosorbent assay (ELISA). Anti-Diphtheria Toxin antibody (3B6) is available as the non-conjugated format. Corynebacterium diphtheriae is a Gram-positive, nonmotile aerobic bacterium commonly found in soil and animal feces, which infects the epithelial cells of the upper respiratory tract. The bacteria produce and secrete a potent toxin that is absorbed and disseminated through lymph channels and blood to susceptible tissues, leading to severe health complications. Diphtheria Toxin (3B6) monoclonal antibody recognizes a protein that plays a critical role in pathogenesis by catalyzing the ADP-ribosylation and inactivation of elongation factor 2 (eEF-2), effectively halting protein synthesis in host cells. The structure of Diphtheria Toxin is particularly important, as Diphtheria Toxin consists of a Y-shaped molecule with three distinct domains: a catalytic domain (fragment A) that exhibits an α + β fold, a transmembrane domain composed of nine α-helices that may facilitate pH-triggered membrane insertion and translocation, and a receptor-binding domain that forms a flattened β-barrel with a jelly-roll-like topology. Together, the transmembrane and receptor-binding domains constitute fragment B, which is essential for the toxin′s entry into host cells. This intricate structure not only underscores the toxin′s mechanism of action but also highlights the potential for therapeutic interventions targeting these domains to mitigate the effects of diphtheria.

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Diphtheria Toxin Antibody (3B6) References:

1. The crystal structure of diphtheria toxin.  |  Choe, S., et al. 1992. Nature. 357: 216-22. PMID: 1589020
2. Diphtheria toxin: mode of action and structure.  |  Collier, RJ. 1975. Bacteriol Rev. 39: 54-85. PMID: 164179
3. Studies on Diphtheria Toxin : II. The Rôle of the Amino Acids in the Metabolism of Bacterium diphtheriae.  |  Davis, L. and Ferry, NS. 1919. J Bacteriol. 4: 217-41. PMID: 16558837
4. Further Studies upon the Effect of Various Carbohydrates on Production of Diphtheria Toxin with Special Reference to its Flocculating Titer and Final pH.  |  Hazen, EL. and Heller, G. 1932. J Bacteriol. 23: 195-209. PMID: 16559546
5. The Purification and Concentration of Diphtheria Toxin: II. Observations on the Nature of the Toxin.  |  Eaton, MD. 1936. J Bacteriol. 31: 367-83. PMID: 16559895
6. The Purification and Concentration of Diphtheria Toxin: III. Separation of Toxin from Bacterial Protein.  |  Eaton, MD. 1937. J Bacteriol. 34: 139-51. PMID: 16560042
7. Studies on Diphtheria Toxin and Its Reaction with Antitoxin.  |  Pappenheimer, AM. 1942. J Bacteriol. 43: 273-89. PMID: 16560498
8. The effect on solutions of diphtheria toxin and diphtheria antitoxin of contact with certain surfaces.  |  Moloney, PJ. and Taylor, EM. 1932. Biochem J. 26: 1754-61. PMID: 16744999
9. Diphtheria toxin.  |  Pappenheimer, AM. 1977. Annu Rev Biochem. 46: 69-94. PMID: 20040
10. Diphtheria toxin forms transmembrane channels in planar lipid bilayers.  |  Donovan, JJ., et al. 1981. Proc Natl Acad Sci U S A. 78: 172-6. PMID: 6264431

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Diphtheria Toxin Antibody (3B6) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Diphtheria Toxin Antibody (3B6) Specifications

Alternate Product Name: Diphtheria Toxin

Product Clone Name: 3B6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Diphtheria Toxin

Epitope Species: Corynebacterium diphtheriae

Product Reactivity/Detection: different determinants of Diphtheria toxin and anatoxin

Species Reactivity/Detection: Corynebacterium diphtheriae

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: ELISA

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Diphtheria Toxin Antibody (3B6): N/A