Diphtheria Toxin A Antibody (7811): sc-57705

Diphtheria Toxin A Antibody (7811) is a mouse monoclonal IgG1 antibody that detects Diphtheria Toxin A from Corynebacterium diphtheriae through enzyme-linked immunosorbent assay (ELISA). Diphtheria Toxin A plays a critical role in the pathogenicity of C. diphtheriae, as Diphtheria Toxin A is responsible for the severe effects of diphtheria, including the inhibition of protein synthesis in host cells. Diphtheria Toxin A achieves this by catalyzing the ADP-ribosylation and subsequent inactivation of elongation factor 2 (eEF-2), which is essential for the translation process in eukaryotic cells. The structure of Diphtheria Toxin A consists of a Y-shaped molecule divided into three distinct domains: a catalytic domain (fragment A) that exhibits an α + β fold, a transmembrane domain composed of nine α-helices that facilitate pH-triggered membrane insertion and translocation, and a receptor-binding domain that forms a flattened β-barrel with a jelly-roll-like topology. The intricate design of these domains allows Diphtheria Toxin A to effectively penetrate host cells and underscores Diphtheria Toxin A′s potential as a target for therapeutic interventions aimed at neutralizing toxic effects. Anti-Diphtheria Toxin A antibody (7811) serves as an invaluable tool for researchers studying the mechanisms of diphtheria and the interactions between Diphtheria Toxin A and host cellular components.

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Diphtheria Toxin A Antibody (7811) References:

1. Understanding the mode of action of diphtheria toxin: a perspective on progress during the 20th century.  |  Collier, RJ. 2001. Toxicon. 39: 1793-803. PMID: 11595641
2. Targeting diphtheria toxin A-chain transcription to activated endothelial cells using an E-selectin promoter.  |  Maxwell, IH., et al. 2003. Angiogenesis. 6: 31-8. PMID: 14517402
3. Cloning, sequence determination, and expression in transfected cells of the coding sequence for the tox 176 attenuated diphtheria toxin A chain.  |  Maxwell, F., et al. 1987. Mol Cell Biol. 7: 1576-9. PMID: 3110596
4. Diphtheria Toxin A-Resistant Cell Lines Enable Robust Production and Evaluation of DTA-Encoding Lentiviruses.  |  Lange, MJ., et al. 2019. Sci Rep. 9: 8985. PMID: 31222087
5. Spatiotemporally controllable diphtheria toxin expression using a light-switchable transgene system combining multifunctional nanoparticle delivery system for targeted melanoma therapy.  |  He, M., et al. 2020. J Control Release. 319: 1-14. PMID: 31838205
6. Generation of a human embryonic stem cell line (SMUDHe010-A-1B) carrying inducible DTA expression cassette in the AAVS1 locus by CRISPR/Cas9-mediated homologous recombination.  |  Qiu, B., et al. 2024. Stem Cell Res. 74: 103283. PMID: 38103335
7. Tat-dependent conditionally replicating adenoviruses expressing diphtheria toxin A for specifically killing HIV-1-infected cells.  |  Ni, F., et al. 2024. Mol Ther. 32: 2316-2327. PMID: 38734901
8. The amino-acid sequence of two non-toxic mutants of diphtheria toxin: CRM45 and CRM197.  |  Giannini, G., et al. 1984. Nucleic Acids Res. 12: 4063-9. PMID: 6427753
9. Farnesylation of CaaX-tagged diphtheria toxin A-fragment as a measure of transfer to the cytosol.  |  Falnes, PO., et al. 1995. Biochemistry. 34: 11152-9. PMID: 7669773
10. Identification of a single amino acid substitution in the diphtheria toxin A chain of CRM 228 responsible for the loss of enzymatic activity.  |  Johnson, VG. and Nicholls, PJ. 1994. J Bacteriol. 176: 4766-9. PMID: 8045910