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Diphenylcarbazone (CAS 538-62-5)

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Alternate Names:
Phenylazoformic acid 2-phenylhydrazide
CAS Number:
538-62-5
Molecular Weight:
240.26
Molecular Formula:
C13H12N4O
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Diphenylcarbazone is a chemical compound that functions as a metal ion indicator in various analytical techniques. It acts as a chelating agent, forming stable complexes with metal ions such as zinc, cadmium, and mercury. The mechanism of action of diphenylcarbazone involves the formation of colored complexes with metal ions, which can be detected and quantified spectrophotometrically. Particularly useful in the determination of metal ions in environmental samples and industrial processes. In the presence of specific metal ions, diphenylcarbazone undergoes a color change, allowing for the sensitive and selective detection of these metal ions. Its ability to form stable complexes with metal ions may be in analytical chemistry for the determination of trace metal concentrations in various matrices.


Diphenylcarbazone (CAS 538-62-5) References

  1. Colorimetric determination of chloride in biological samples by using mercuric nitrate and diphenylcarbazone.  |  Yokoi, K. 2002. Biol Trace Elem Res. 85: 87-94. PMID: 11881802
  2. [Application of diphenylcarbazone in colorimetric microdetermination of copper in food products and in ready foodstuffs].  |  LAPIN, LN. and PRIEV, IG. 1958. Vopr Pitan. 17: 68-72. PMID: 13558031
  3. Preparation of the diphenylcarbazone-functionalized silica gel and its application to on-line selective solid-phase extraction and determination of mercury by flow-injection spectrophotometry.  |  Fan, J., et al. 2008. J Hazard Mater. 150: 343-50. PMID: 17560020
  4. Spectrophotometric determination of ziram, ferbam and zineb with diphenylcarbazone.  |  Malik, AK. and Rao, AL. 1991. Talanta. 38: 941-4. PMID: 18965241
  5. The accurate volumetric determination of zinc and nickel using diphenylcarbazone as indicator.  |  EVANS, BS. 1946. Analyst. 71: 455-9. PMID: 20281597
  6. Development of a new chromium reducing antioxidant capacity (CHROMAC) assay for plants and fruits.  |  Işık, E., et al. 2013. Talanta. 111: 119-24. PMID: 23622534
  7. In-syringe magnetic-stirring-assisted liquid-liquid microextraction for the spectrophotometric determination of Cr(VI) in waters.  |  Henríquez, C., et al. 2013. Anal Bioanal Chem. 405: 6761-9. PMID: 23812854
  8. Solid Phase Extraction of Trace Elements in Waterand Tissue Samples on a Mini Column with Diphenylcarbazone Impregnated Nano-TiO2 and Their Determination by Inductively Coupled Plasma Optical Emission Spectrometry.  |  Baytak, S. and Arslan, Z. 2015. Clean (Weinh). 43: 822-829. PMID: 26236403
  9. Quantification of Hexavalent Chromium in Surface Water Samples by a Selective Electrochemical Method.  |  Baig, JA., et al. 2018. J AOAC Int. 101: 577-586. PMID: 28838331
  10. Development of an Accurate and Sensitive Analytical Method for the Determination of Cadmium at Trace Levels Using Dispersive Liquid-Liquid Microextraction Based on the Solidification of Floating Organic Drops Combined with Slotted Quartz Tube Flame Atomic Absorption Spectrometry.  |  Aydin, I., et al. 2018. J AOAC Int. 101: 843-847. PMID: 28903806
  11. Low-Turn-On-Voltage, High-Brightness, and Deep-Red Light-Emitting Electrochemical Cell Based on a New Blend of [Ru(bpy)3]2+ and Zn-Diphenylcarbazone.  |  Shahroosvand, H., et al. 2018. ACS Omega. 3: 9981-9988. PMID: 31459126
  12. Molecularly engineered electroplex emission for an efficient near-infrared light-emitting electrochemical cell (NIR-LEC).  |  Shahroosvand, H., et al. 2020. RSC Adv. 10: 14099-14106. PMID: 35498498
  13. The use of diphenylcarbazone for the determination of microgram amounts of lead.  |  Trinder, N. 1966. Analyst. 91: 587-90. PMID: 5914300
  14. A sensitive method for the determination of free fatty acids in plasma.  |  Hron, WT. and Menahan, LA. 1981. J Lipid Res. 22: 377-81. PMID: 7240964

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Diphenylcarbazone, 10 g

sc-252751
10 g
$84.00

Diphenylcarbazone, 25 g

sc-252751B
25 g
$119.00

Diphenylcarbazone, 50 g

sc-252751A
50 g
$187.00