DIP2C Double Nickase Plasmid (h): sc-411642-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DIP2C Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DIP2C Double Nickase Plasmid (h) and DIP2C Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DIP2C. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DIP2C Double Nickase Plasmid (h) | sc-411642-NIC | 20 µg | $410.00 | |||
DIP2C Double Nickase Plasmid (h2) | sc-411642-NIC-2 | 20 µg | $410.00 |
DIP2C (disco-interacting protein 2 homolog C) encodes a nuclear protein implicated in chromatin-associated regulation of gene expression and cellular differentiation programs. Reported functional studies link DIP2 family proteins to DNA methylation-associated processes and neuronal development pathways, suggesting roles in maintaining transcriptional states during cell fate decisions. Altered DIP2C expression and genomic perturbations have been observed in multiple tumor datasets, supporting its investigation in cancer biology, genome stability, and epigenetic dysregulation. As a result, DIP2C is frequently studied in models of proliferation control, lineage commitment, and transcriptional network remodeling.
DIP2C Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DIP2C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DIP2C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DIP2C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DIP2C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.