Dimethyl Sulfoxide (DMSO), cell culture reagentA polar, aprotic organic solvent ideal for cryopreservation

Dimethyl Sulfoxide (DMSO), cell culture reagent (CAS 67-68-5)

Dimethyl Sulfoxide (DMSO), cell culture reagent | CAS 67-68-5 is rated 5.0 out of 5 by 4.
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Synonym: methylsulfinylmethane
Application: A polar, aprotic organic solvent ideal for cryopreservation
CAS Number: 67-68-5
Purity: ≥99%
Molecular Weight: 78.13
Molecular Formula: C2H6OS
* Refer to Certificate of Analysis for lot specific data (including water content).
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Dimethyl Sulfoxide (DMSO), cell culture reagent is ideal for cryopreservation. DMSO is a polar, aprotic organic solvent with many applications in chemical and biological research. DMSO can uniquely provide solubility to certain very polar small molecules, such as simple biomolecules with multiple protic functional groups. It can also be used as an oxidation reagent in certain transformations. The yield of PCR processes is amplified with DMSO as a co-solvent, helping to inhibit the formation of secondary structures from DNA fragments.


References

1. Sweat, F.W. and Epstein, W.W. 1967. J. Org. Chem. 32: 835-838. PMID: 6042131
2. Winship, P.R. 1989. Nucleic Acids Res. 17: 1266. PMID: 2922271
3. Chakrabarti, R. and Schutt, C.E. 2001. Gene. 274: 293-298. PMID: 11675022
4. Heidemann, R., et al. 2010. Biotechnol. Prog. 26: 1154-1163. PMID: 20730770
5. Thirumala, S., et al. 2010. Stem Cells Dev. 19: 513-522. PMID: 19788372

Usage :
Dimethyl Sulfoxide (DMSO), Cell Culture reagent is used in molecular biology, especially in the polymerase chain reaction (PCR), cell lysis, in transformation and transfection and in cytofluorimetric assessment. DMSO is most often used at a concentration of 10%.
Physical State :
Liquid
Solubility :
Soluble in water, methanol, acetone, ether, chloroform, benzene, and ethanol. DMSO is miscible in most of the common organic solvents such as alcohols, esters, ketones, ethers, chlorinated solvents and aromatics.
Storage :
Desiccate at room temperature
Melting Point :
16-19° C (lit.)
Boiling Point :
189° C at 101.3 kPa
Density :
1.10 g/mL(lit.)
Refractive Index :
n20D 1.48 (lit.)
IC50 :
HII4E cells : IC50 = 199 µg.mL-1 (mouse)
Ki Data :
Choline acetylase: Ki= 25 mM (rat)
pK Values :
pKa: -4.54 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
PV6210000
PubChem CID :
679
Merck Index :
14: 3259
MDL Number :
MFCD00002089
EC Number :
200-664-3
Beilstein Registry :
506008
SMILES :
CS(=O)C

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Certificate of Analysis

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Dimethyl Sulfoxide (DMSO), cell culture reagent  Product Citations

See how others have used Dimethyl Sulfoxide (DMSO), cell culture reagent. Click on the entry to view the PubMed entry .

Citations 1 to 8 of 8 total

PMID: # 31031137  Kalkan, T.|Bornelöv, S.|Mulas, C.|Diamanti, E.|Lohoff, T.|Ralser, M.|Middelkamp, S.|Lombard, P.|Nichols, J.|Smith, A.| et al. 2019. Cell Stem Cell. 24: 785-801.e7.

PMID: # 31249607  Li, J.|Dang, N.|Martinez-Lopez, N.|Jowsey, PA.|Huang, D.|Lightowlers, RN.|Gao, F.|Huang, JY.| et al. 2019. Cell Div. 14: 5.

PMID: # 31068616  Myszor, IT.|Parveen, Z.|Ottosson, H.|Bergman, P.|Agerberth, B.|Strömberg, R.|Gudmundsson, GH.| et al. 2019. Sci Rep. 9: 7114.

PMID: # 29556174  Li, X.|Peng, J.|Pang, J.|Wu, Y.|Huang, X.|Li, Y.|Zhou, J.|Gu, L.|Sun, X.|Chen, L.|Vitek, MP.|Jiang, Y.| et al. 2018. Front Neurosci. 12: 127.

PMID: # 29163636  House, JS. et al. 2017. Front Genet. 8: 168.

PMID: # 25010905  2014. Nat Protoc. 9: 1848-59.

PMID: # 24375454  2014. Eur. J. Immunol. 44: 1108-18.

PMID: # 22704420  2012. Zhong Xi Yi Jie He Xue Bao. 10: 695-700.

Citations 1 to 8 of 8 total

Can it be used for freezing astrocytes and human endothelial cells. Does it come filter sterilized.

Asked by: Sana S
Thank you for your question. This is cell culture grade DMSO. Unfortunately we do not specify how the product is sterilized, but as indicated on available lot-specific CoAs, the product passes sterility test. Please contact our Technical Service to request a sample CoA from a current or recent lot.
Answered by: Technical Support
Date published: 2019-08-20

Cuál es el formato del envase? cristal o plástico?

Asked by: RVirievac
Thank you for your question. This biochemical is provided as a liquid in a glass vial.
Answered by: Tech Service
Date published: 2019-04-09

Dear All, I need your offer for 3x100ml? 1And information about transport costs to Serbia. Best regards, Mrs. Dragica Grudic

Asked by: Dragica grudic
The lead time and the shipping costs depend on the destination. For more information please contact costumer care by phone or by live chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Hi, We want to order DMSO (undiluted-100%) for cell culture. I want to know if it has been tested for low endo-toxins. Thanks.

Asked by: Harleen
Thank you for your question. Yes, endotoxin levels are tested for this cell culture grade DMSO. The specifications are provided on our CoA. Each lot of this chemical comes with a lot-specific Certificate Of Analysis. You may request a lot-specific Certificate Of Analysis on our website. Alternatively, please contact our Technical Service Department to request a copy of the CoA for our most recent lot.
Answered by: Technical Support
Date published: 2018-06-05

Is this product sterilized?

Asked by: zhangmeng
Thank you for your question. This product is not sterilely filtered. It is cell culture grade and due to the toxicity of the solvent, not much will grow in it. You can sterile filter the item in the pure form using a nitrocellulose filter or using a regular filter when the DMSO is diluted. We do offer DMSO sterile filtered (see sc-359032). If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-09-14

What are the endotoxin levels in this chemical?

Asked by: Jz28sail
Thank you for your question. Endotoxin levels for sc-358801, Dimethyl Sulfoxide (DMSO), cell culture reagent are expected to be <2.5 EU/mL.
Answered by: Chemical Support 5
Date published: 2017-04-13
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Rated 5 out of 5 by from Great features I bought Dimethyl Sulfoxide (DMSO), cell culture reagent, 100 ml from SCBT about 2 weeks ago. It works well in our experiments.
Date published: 2019-08-22
Rated 5 out of 5 by from GOOD FOR CELL CULTURE We purchased for cell culture experiments and works good.
Date published: 2017-05-10
Rated 5 out of 5 by from Bought it for cell culture Bought it for cell culture, worked fine.
Date published: 2015-06-12
Rated 5 out of 5 by from Worked well in cell culture Worked well in cell culture, use it routinely.
Date published: 2015-04-24
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