Digoxigenin NHS ester (CAS 129273-26-3)

Digoxigenin NHS ester is a specialized derivative of the steroid digoxigenin, which is naturally occurring and found in plants of the genus Digitalis. The term ′NHS ester′ refers to the compound incorporating an N-hydroxysuccinimide ester group, which is used in biochemistry to label or modify proteins and other molecules. The structure of digoxigenin NHS ester is characterized by a steroidal core, typical of cardenolides like digoxigenin, with a hydrophobic multi-ring structure. Attached to this core is an ester linkage connecting the steroid to N-hydroxysuccinimide (NHS). The NHS ester group is well-known for its reactivity towards primary amines. This makes digoxigenin NHS ester an ideal compound for labeling proteins, as proteins typically have multiple amine groups that are available for reaction. In terms of its physical properties, digoxigenin NHS ester is typically a white or off-white solid. Due to its reactivity, it is often stored in a freezer and handled under conditions that minimize exposure to moisture and air. Its main use is in the field of biochemistry, where it serves as useful for labeling and tracking proteins and other biomolecules in various research applications.