dHAND Antibody (A-12): sc-398167

dHAND Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects dHAND protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-dHAND antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. dHAND, also known as HAND2, is a crucial transcription factor that plays a significant role in cardiac development, particularly in establishing left-right asymmetry in the heart. dHAND is predominantly expressed on the right side of the looped heart tube and in the pulmonary ventricle, where dHAND activates the transcription of essential genes such as Ufd1, which is involved in the ubiquitin-proteasome pathway, and Cdc45, a key player in DNA replication. Proper functioning of dHAND is vital for the development of the heart′s structure, ensuring correct formation of heart chambers during cardiogenesis. dHAND is expressed in sympathetic neurons and chromaffin cells throughout embryonic and fetal development, highlighting dHAND′s importance in neural crest development. Interaction between dHAND and eHAND (HAND1) further underscores the complexity of cardiac morphogenesis, as these proteins coordinate the development of distinct segments of the heart tube, ultimately leading to the formation of a functional heart.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

dHAND Antibody (A-12) References:

1. A molecular pathway revealing a genetic basis for human cardiac and craniofacial defects.  |  Yamagishi, H., et al. 1999. Science. 283: 1158-61. PMID: 10024240
2. HAND proteins: molecular mediators of cardiac development and congenital heart disease.  |  Srivastava, D. 1999. Trends Cardiovasc Med. 9: 11-8. PMID: 10189962
3. Role of dHAND in the anterior-posterior polarization of the limb bud: implications for the Sonic hedgehog pathway.  |  Fernandez-Teran, M., et al. 2000. Development. 127: 2133-42. PMID: 10769237
4. Human eHAND, but not dHAND, is down-regulated in cardiomyopathies.  |  Natarajan, A., et al. 2001. J Mol Cell Cardiol. 33: 1607-14. PMID: 11549340
5. Misexpression of dHAND induces ectopic digits in the developing limb bud in the absence of direct DNA binding.  |  McFadden, DG., et al. 2002. Development. 129: 3077-88. PMID: 12070084
6. Targeted deletion of a branchial arch-specific enhancer reveals a role of dHAND in craniofacial development.  |  Yanagisawa, H., et al. 2003. Development. 130: 1069-78. PMID: 12571099
7. Phox2 and dHAND transcription factors select shared and unique target genes in the noradrenergic cell type.  |  Rychlik, JL., et al. 2005. J Mol Neurosci. 27: 281-92. PMID: 16280598
8. A subclass of bHLH proteins required for cardiac morphogenesis.  |  Srivastava, D., et al. 1995. Science. 270: 1995-9. PMID: 8533092
9. Regulation of cardiac mesodermal and neural crest development by the bHLH transcription factor, dHAND.  |  Srivastava, D., et al. 1997. Nat Genet. 16: 154-60. PMID: 9171826
10. The bHLH factors, dHAND and eHAND, specify pulmonary and systemic cardiac ventricles independent of left-right sidedness.  |  Thomas, T., et al. 1998. Dev Biol. 196: 228-36. PMID: 9576835
11. A signaling cascade involving endothelin-1, dHAND and msx1 regulates development of neural-crest-derived branchial arch mesenchyme.  |  Thomas, T., et al. 1998. Development. 125: 3005-14. PMID: 9671575
12. Molecular cloning of the human Hand1 gene/cDNA and its tissue-restricted expression in cytotrophoblastic cells and heart.  |  Knöfler, M., et al. 1998. Gene. 224: 77-86. PMID: 9931445

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-dHAND Antibody (A-12) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-dHAND Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

dHAND Antibody (A-12) Specifications

Alternate Product Name: dHAND

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-110

Epitope Target: dHAND

Epitope Species: human

Product Reactivity/Detection: dHAND

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for dHAND Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent