Molecular structure of DEPMPO, CAS Number: 157230-67-6
DEPMPO (CAS 157230-67-6)
See product citations (7)
Alternate Names:
DEPMPO is also known as 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide.
Application:
DEPMPO is an efficient spin trap for in-vitro superoxide detection of O-, N-, S- and C-centered radicals.
CAS Number:
157230-67-6
Purity:
≥98%
Molecular Weight:
235.21
Molecular Formula:
C9H18NO4P
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
DEPMPO is a phosphorylated derivative of the popular DMPO spin trap. DEPMPO has a longer life-time than DMPO. DEPMPO is the most efficient spin trap for the in vitro detection of O-, N-, S- and C-centered free radicals. It is possible for DEPMPO to distinguish between superoxide-dependent and independent mechanisms that lead to the hydroxyl radical. DEPMPO is less lipophilic (Kp=0.16) than DIPPMPO. DEPMPO works in concentrations as low as 1 mM, compared to DMPO which works at around 5 mM. DMPO also produces OH adducts, while DEPMPO may trap superoxide radicals without any OH adduct produced during decomposition.
DEPMPO (CAS 157230-67-6) References
- Improved method for EPR detection of DEPMPO-superoxide radicals by liquid nitrogen freezing. | Dambrova, M., et al. 2000. Biochem Biophys Res Commun. 275: 895-8. PMID: 10973818
- Spin trapping of lipid radicals with DEPMPO-derived spin traps: detection of superoxide, alkyl and alkoxyl radicals in aqueous and lipid phase. | Stolze, K., et al. 2000. Free Radic Biol Med. 29: 1005-14. PMID: 11084289
- Detection and removal of contaminating hydroxylamines from the spin trap DEPMPO, and re-evaluation of its use to indicate nitrone radical cation formation and S(N)1 reactions. | Jackson, SK., et al. 2002. Free Radic Biol Med. 32: 228-32. PMID: 11827748
- Nonradical mechanism of (bi)sulfite reaction with DEPMPO: cautionary note for SO3*- radical spin trapping. | Potapenko, DI., et al. 2003. Free Radic Biol Med. 34: 196-206. PMID: 12521601
- Characterization of the high resolution ESR spectra of the methoxyl radical adducts of 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO). | Dikalov, S., et al. 2003. Free Radic Res. 37: 705-12. PMID: 12911266
- Characterization of the high-resolution ESR spectra of superoxide radical adducts of 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Analysis of conformational exchange. | Dikalov, S., et al. 2005. Free Radic Res. 39: 825-36. PMID: 16036362
- Synthesis and spin-trapping behavior of 5-ChEPMPO, a cholesteryl ester analogue of the spin trap DEPMPO. | Hardy, M., et al. 2005. J Org Chem. 70: 10426-33. PMID: 16323853
- The line asymmetry of electron spin resonance spectra as a tool to determine the cis:trans ratio for spin-trapping adducts of chiral pyrrolines N-oxides: the mechanism of formation of hydroxyl radical adducts of EMPO, DEPMPO, and DIPPMPO in the ischemic-reperfused rat liver. | Culcasi, M., et al. 2006. Free Radic Biol Med. 40: 1524-38. PMID: 16632113
- Synthesis and characterization of a practically better DEPMPO-type spin trap, 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO). | Kamibayashi, M., et al. 2006. Free Radic Res. 40: 1166-72. PMID: 17050170
- Early specific free radical-related cytotoxicity of gas phase cigarette smoke and its paradoxical temporary inhibition by tar: An electron paramagnetic resonance study with the spin trap DEPMPO. | Culcasi, M., et al. 2006. Chem Biol Interact. 164: 215-31. PMID: 17083924
- Design of new derivatives of nitrone DEPMPO functionalized at C-4 for further specific applications in superoxide radical detection. | Chalier, F., et al. 2007. J Org Chem. 72: 7886-92. PMID: 17880238
- EPR spin-trapping and nano LC MS/MS techniques for DEPMPO/OOH and immunospin-trapping with anti-DMPO antibody in mitochondrial electron transfer system. | Chen, YR. 2008. Methods Mol Biol. 477: 75-88. PMID: 19082940
- Identification of free radicals by spin trapping with DEPMPO and MCPIO using tandem mass spectrometry. | Reis, A., et al. 2009. Eur J Mass Spectrom (Chichester). 15: 689-703. PMID: 19940335
- DEPMPO: an efficient tool for the coupled ESR-spin trapping of alkylperoxyl radicals in water. | Karoui, H., et al. 2011. Org Biomol Chem. 9: 2473-80. PMID: 21347497
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DEPMPO, 50 mg | sc-202132 | 50 mg | $193.00 | |||
DEPMPO, 500 mg | sc-202132A | 500 mg | $1155.00 |