Anti-DENR Antibody (NQ-B8) recommended for detection of DENR of mouse, rat and human origin by WB, IP and ELISA

Anti-DENR Antibody (NQ-B8): sc-134315

Anti-DENR Antibody (NQ-B8) is rated 5.0 out of 5 by 1.
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Datasheets

    • DENR Antibody (NQ-B8) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 100 µg/ml
    • raised against recombinant DENR protein of human origin
    • recommended for detection of DENR of mouse, rat and human origin by WB, IP and ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DENR Antibody (NQ-B8). This work is in progress.
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Hello! I'm interested in this antibody. Why is the band expected at ~28kd, but DENR is a 22kd protein? 

Asked by: YLfhcrc
Thank you for your question. The predicted molecular weight of DENR is 22 kDa, however the observed molecular weight of the endogenous protein is ~27-31 kDa in the cell lines we have tested. The difference between the predicted and observed molecular weight may be due to post-translational modifications of the protein.
Answered by: Technical Support
Date published: 2019-03-10

For Western Blot, is it recommended to use denatured or non-denatured conditions with DENR (NQ-B8): sc-134315 antibody?

Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from WB publishable data with mouse aorta WB publishable data with mouse aorta - SCBT Publication Review
Date published: 2014-10-01
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