Deltex-1 CRISPR/Cas9 KO Plasmid (m): sc-420429
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Deltex-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Deltex-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Deltex-1 CRISPR/Cas9 KO Plasmid (m) | sc-420429 | 20 µg | $397.00 |
Overview
Dtx1 encodes Deltex-1, an E3 ubiquitin ligase that functions as a context-dependent modulator of Notch signaling by influencing receptor-proximal signaling complexes and turnover of pathway components. Through ubiquitin-dependent regulation, Deltex-1 can shape transcriptional outputs that affect cell fate decisions, differentiation programs, and immune cell homeostasis in mouse models. Dtx1 activity has been linked to pathways controlling proteostasis and signal transduction, including cross-talk with NF-κB–associated inflammatory programs in certain cellular settings. Dysregulated Notch/ubiquitin signaling involving Deltex-1 has been studied in immune dysfunction and oncogenic processes, making Dtx1 a useful node for mechanistic dissection of pathway-dependent phenotypes.
Deltex-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dtx1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dtx1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dtx1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Deltex-1 protein expression.
This CRISPR knockout system enables efficient generation of Dtx1-deficient cell models for investigation of Deltex-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dtx1 exon(s) critical for Deltex-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dtx1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Deltex-1 CRISPR/Cas9 KO Plasmid (m) and Deltex-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dtx1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Deltex-1 HDR Plasmid (m) and Deltex-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dtx1 homology arms to support homology-directed repair at defined Dtx1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.