Dectin-1 Antibody (15Y9): sc-73897

Dectin-1 Antibody (15Y9) is a rat monoclonal IgG2b antibody that detects Dectin-1 protein of mouse origin by western blotting (WB). Anti-Dectin-1 antibody (15Y9) is available as non-conjugated format. Dectin-1, also known as CLEC7A, is a crucial type II transmembrane receptor that plays a significant role in immune response by recognizing beta-glucans, polysaccharides found in cell walls of fungi and certain bacteria. This recognition is vital for activation of innate immune cells, particularly macrophages, which utilize Dectin-1 to identify and respond to fungal infections. Dectin-1 features a single extracellular carbohydrate recognition domain and an immunoreceptor tyrosine activation motif in cytoplasmic tail, facilitating signal transduction upon ligand binding. Dectin-1 exists in two major isoforms, A and B, which differ by presence of a stalk region that separates carbohydrate recognition domain from transmembrane region. Dectin-1′s ability to bind both endogenous and exogenous ligands, including T-lymphocytes, underscores importance in modulating immune responses. Notably, Dectin-1B is expressed on various dendritic cell subpopulations during development from peripheral blood monocytes and is present on myeloid cell populations, such as monocytes, macrophages, and neutrophils. Given pivotal role in immune recognition and response, Dectin-1 serves as a valuable target for research into immunomodulatory properties of beta-glucans, paving way for potential therapeutic applications in treating infections and enhancing immune function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Dectin-1 Antibody (15Y9) References:

1. Characterization of the human beta -glucan receptor and its alternatively spliced isoforms.  |  Willment, JA., et al. 2001. J Biol Chem. 276: 43818-23. PMID: 11567029
2. Dectin-1 is a major beta-glucan receptor on macrophages.  |  Brown, GD., et al. 2002. J Exp Med. 196: 407-12. PMID: 12163569
3. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.  |  Taylor, PR., et al. 2002. J Immunol. 169: 3876-82. PMID: 12244185
4. Molecular and functional characterization of human Dectin-1.  |  Grünebach, F., et al. 2002. Exp Hematol. 30: 1309-15. PMID: 12423684
5. Pattern recognition receptors: doubling up for the innate immune response.  |  Gordon, S. 2002. Cell. 111: 927-30. PMID: 12507420
6. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance.  |  Daley, D., et al. 2017. Nat Med. 23: 556-567. PMID: 28394331
7. Dectin-1 Contributes to Myocardial Ischemia/Reperfusion Injury by Regulating Macrophage Polarization and Neutrophil Infiltration.  |  Fan, Q., et al. 2019. Circulation. 139: 663-678. PMID: 30586706
8. The macrophage tetraspan MS4A4A enhances dectin-1-dependent NK cell-mediated resistance to metastasis.  |  Mattiola, I., et al. 2019. Nat Immunol. 20: 1012-1022. PMID: 31263276
9. Galectin 3 enhances platelet aggregation and thrombosis via Dectin-1 activation: a translational study.  |  Chen, Y., et al. 2022. Eur Heart J. 43: 3556-3574. PMID: 35165707
10. Blocking Dectin-1 prevents colorectal tumorigenesis by suppressing prostaglandin E2 production in myeloid-derived suppressor cells and enhancing IL-22 binding protein expression.  |  Tang, C., et al. 2023. Nat Commun. 14: 1493. PMID: 36932082
11. Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion.  |  Ye, S., et al. 2023. Cell Mol Life Sci. 80: 184. PMID: 37340199
12. Cancer immunotherapy via synergistic coactivation of myeloid receptors CD40 and Dectin-1.  |  Wattenberg, MM., et al. 2023. Sci Immunol. 8: eadj5097. PMID: 37976347