Decanoyl coenzyme A monohydrate (CAS 1264-57-9)

Decanoyl coenzyme A monohydrate is a vital intermediate in fatty acid metabolism and is extensively used in biochemical research to study metabolic pathways and enzyme activities. This compound, consisting of a decanoic acid moiety linked to coenzyme A, plays a crucial role in the β-oxidation pathway, where it is involved in the degradation of fatty acids to generate acetyl-CoA, a key substrate for the citric acid cycle. Researchers utilize decanoyl coenzyme A to investigate the catalytic mechanisms and substrate specificity of various acyl-CoA dehydrogenases and thiolases, which are enzymes essential for fatty acid catabolism. In addition to its role in β-oxidation, decanoyl coenzyme A monohydrate is used to study the regulation of lipid biosynthesis. It serves as a substrate in assays designed to measure the activity of enzymes like fatty acid synthase and elongases, which are involved in the synthesis and elongation of fatty acids. This compound is also employed in studies examining the interaction between acyl-CoA molecules and proteins involved in lipid transport and signaling, such as acyl-CoA binding proteins and peroxisome proliferator-activated receptors (PPARs). Furthermore, decanoyl coenzyme A is utilized in structural biology to explain the conformational changes and binding interactions of enzymes and proteins with acyl-CoA substrates. These studies provide significant insights into the molecular mechanisms regulating fatty acid metabolism and the broader implications for energy homeostasis and cellular function.