Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DEC2 Double Nickase Plasmid (m): sc-429771-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DEC2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DEC2 Double Nickase Plasmid (m) and DEC2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Bhlhe41. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DEC2 Double Nickase Plasmid (m) | sc-429771-NIC | 20 µg | $410.00 | |||
DEC2 Double Nickase Plasmid (m2) | sc-429771-NIC-2 | 20 µg | $410.00 |
Mouse Bhlhe41 encodes the basic helix–loop–helix transcription factor DEC2, a context-dependent regulator of gene expression that integrates circadian timing with cellular differentiation programs. DEC2 participates in transcriptional networks downstream of core clock machinery and modulates processes such as sleep–wake regulation, metabolic homeostasis, and lineage-specific transcriptional repression/activation. In immune and stromal contexts, BHLHE41/DEC2 has been linked to control of cytokine programs and hypoxia-associated transcriptional responses, connecting it to pathways that shape inflammation and tissue adaptation. Dysregulated DEC2 activity has been associated with altered sleep phenotypes and has been studied in models of metabolic and immune-related disease states where circadian transcription influences cellular function.
DEC2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Bhlhe41 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Bhlhe41. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Bhlhe41 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Bhlhe41-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.