DEC1 Double Nickase Plasmid (h): sc-402058-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DEC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DEC1 Double Nickase Plasmid (h) and DEC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BHLHE40. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DEC1 Antibody (S-8): sc-101023
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DEC1 Double Nickase Plasmid (h) | sc-402058-NIC | 20 µg | $410.00 | |||
DEC1 Double Nickase Plasmid (h2) | sc-402058-NIC-2 | 20 µg | $410.00 |
BHLHE40 encodes the basic helix–loop–helix transcription factor DEC1 (also known as BHLHE40/STRA13), a context-dependent regulator of gene expression that integrates hypoxia and circadian inputs. DEC1 participates in transcriptional programs downstream of oxygen sensing and cellular stress, influencing cell-cycle progression, differentiation, and metabolic adaptation through interactions with core clock components and hypoxia-responsive networks. By modulating targets involved in proliferation, apoptosis, and inflammatory signaling, BHLHE40 has been associated with altered tumor biology, immune cell function, and responses to microenvironmental stress. These properties make DEC1 a useful node for studying transcriptional control in hypoxia–circadian crosstalk and stress-adaptive pathways in human cells.
DEC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BHLHE40 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BHLHE40. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BHLHE40 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BHLHE40-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.