DEAE-Cellulose (CAS 9013-34-7)

Usage :

Provided as a dry resin.
The following procedure should be carried out in a Buchner funnel.
•Suspend dry resin in 5 volumes diH2O and allow to swell and settle for 30-45 minutes. Measure volume of settled resin to determine Column Volume (CV) for washing steps.


**If needed, use a homogenizer to break up any aggregated particles present in the suspension.


For activation/regeneration:

•Filter suspension and re-suspend resin in 2 CV of 0.1M NaOH/0.5M NaCl for 10 minutes. Pour slurry into Buchner funnel and allow buffer to slowly drain off (~1 V buffer in 5 minutes).

•Repeat using 0.5M NaCl (without 0.1M NaOH)

•Repeat using 0.1M HCl/0.5 M NaCl

•Repeat using diH2O

•Continue washing with diH2O until effluent pH is 5 or greater (~5-10 CV).

•Suspend the resin in 2 CV of 1M NaCl and adjust pH to 7-8 with NaOH and store at 4°C.

To Use Resin:

•Filter resin and pass 5 CV of diH2O through resin.

•Resuspend in 2 CV of 10X buffer of your choice and filter.

•Remove resin from funnel and re-suspend with 5 CV of 1X buffer and filter. Wash with 1X buffer until pH is within 0.15 pH units of the 1X buffer.

•Pack resin into column via gravity flow.

•Apply sample, wash resin, and elute sample.

Physical State :

Solid

Storage :

Store at 4° C

WGK Germany :

3

MDL Number :

MFCD00146622