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Ac-lysine (AKL5C1): sc-32268. Western blot analysis of lysine acetylation in untreated (A) and deacetylase inhibitor cockt
Ac-lysine (AKL5C1): sc-32268. Western blot analysis of lysine acetylation in untreated (A) and deacetylase inhibitor cocktail (sc-362323) treated (B) NIH/3T3 whole cell lysates.
Ac-lysine (AKL5C1): sc-32268. Western blot analysis of lysine acetylation in untreated (A) and deacetylase inhibitor cockt
Ac-lysine (AKL5C1): sc-32268. Western blot analysis of lysine acetylation in untreated (A) and deacetylase inhibitor cocktail (sc-362323) treated (B) NIH/3T3 whole cell lysates.
Ac-lysine (7F8): sc-81623. Western blot analysis of lysine acetylation in untreated (A) and deacetylase inhibitor cocktail
Ac-lysine (7F8): sc-81623. Western blot analysis of lysine acetylation in untreated (A) and deacetylase inhibitor cocktail (sc-362323) treated (B) NIH/3T3 whole cell lysates.
Western blot analysis of α Tubulin acetylation in untreated (A,C) and deacetylase inhibitor cocktail (sc-362323) treated (
Western blot analysis of α Tubulin acetylation in untreated (A,C) and deacetylase inhibitor cocktail (sc-362323) treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include acetylated α Tubulin (6-11B-1): sc-23950 (A,B) and α Tubulin (DM1A): sc-32293 (C,D).
Ac-lysine (AKL5C1): sc-32268. Western blot analysis of lysine acetylation in untreated (A) and deacetylase inhibitor cocktail (sc-362323) treated (B) NIH/3T3 whole cell lysates.

Deacetylation Inhibition Cocktail

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Alternate Names:
Deacetylation Inhibition Cocktail also known as Deacetylation Inhibition
Application:
Deacetylation Inhibition Cocktail is preserves the acetylation state of acetyl-lysine modified proteins
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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SDS & Certificate of Analysis

Deacetylation Inhibition Cocktail is a synergistic combination of chemicals designed to preserve the acetylation state of proteins including histone, tubulin, p53, and other acetyl-lysine modified targets. Lysine acetylation is an important post-translational modification that can affect protein transcription, cell cycle, and signal transduction pathways. The balance between acetyltransferase enzymes and deacetylase enzymes affects chromatin condensation, transcription factor activity and protein aggregation and is of significant interest to researchers. Deacetylation Inhibition Cocktail is a useful tool for investigating these important biological pathways.
Deacetylation Inhibition Cocktail has been developed and tested in our laboratories and has shown to effectively inhibit HDAC′s and Sirtuins in various cellular samples and applications.
Suitable for use in Western Blotting, Flow Cytometry, and ImmunoFluorescence applications.


Deacetylation Inhibition Cocktail References

  1. Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage.  |  Solomon, JM., et al. 2006. Mol Cell Biol. 26: 28-38. PMID: 16354677
  2. Acetylation modulates prolactin receptor dimerization.  |  Ma, L., et al. 2010. Proc Natl Acad Sci U S A. 107: 19314-9. PMID: 20962278
  3. Acetylation-deacetylation of the transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) regulates its transcriptional activity and nucleocytoplasmic localization.  |  Kawai, Y., et al. 2011. J Biol Chem. 286: 7629-40. PMID: 21196497
  4. Different effects of histone deacetylase inhibitors nicotinamide and trichostatin A (TSA) in C17.2 neural stem cells.  |  Wang, H., et al. 2012. J Neural Transm (Vienna). 119: 1307-15. PMID: 22407380
  5. NAD+-boosting therapy alleviates nonalcoholic fatty liver disease via stimulating a novel exerkine Fndc5/irisin.  |  Li, DJ., et al. 2021. Theranostics. 11: 4381-4402. PMID: 33754067

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Product NameCatalog #UNITPriceQtyFAVORITES

Deacetylation Inhibition Cocktail, 2 ml

sc-362323
2 ml
$58.00

Could this reagent (sc-362323) be use in a buffer lysis for protein extraction such as RIPA and if it is possible, how it should be used?

Asked by: Dan92
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-07-22

Hello, You write that 2ml of this cocktail is enough to treat 200ml of culture media. Does that mean you recommend a 1:100 dilution of the cocktail? Can you please specify the concentration range that you tried and worked? And what cells you used?

Asked by: Georgiana
Thank you for your question. We have specifically tested this in NIH/3T3 whole cell lysate. Recommended induction times of 16-48 hours. Since applications vary, the optimal working dilution should be determined by the researcher.
Answered by: Technical Support
Date published: 2024-05-22

I want to know if I can use this cocktail to inhibit deacetylation in cell extracts with non denatured proteins. My interest is to use in extracts that I'll immunoprecipitate. Do you think that 1h incubation is enough to inhibit deacetylases?

Asked by: Raphael Pavani
Thank you for your question. Yes, this is a suitable application for our Deacetylation Inhibition Cocktail: sc-362323. We recommend induction times of 16-48 hours. Since applications vary, the optimal working dilution should be determined by the researcher.
Answered by: Technical Support
Date published: 2021-06-22

My question was regarding the amount deacetylation cocktail to add for making tissue lysates for WB. Your answer: We recommend 40–60 µg whole cell lysate for Western Blot. That was not what I had asked. The amount of this cocktail to add,

Asked by: spar
Thank you for your question. This product, Deacetylation Inhibition Cocktail: sc-362323, is provided in 2 ml DMSO and is sufficient to treat 200 ml of culture media. We recommend induction times of 16-48 hours. Since applications vary, the optimal working dilution should be determined by the researcher.
Answered by: Technical Support
Date published: 2024-11-15

What is the recommended volume when making tissue lysates for Western blots?

Asked by: spar
Thank you for your question. We recommend 40–60 µg whole cell lysate for Western Blot. Please refer to the protocol of Western Blot for more details: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 12
Date published: 2021-06-22

Will this product work for purified protein sample?

Asked by: two2igm05
Thank you for your question. The components of the Deacetylation Inhibition Cocktail, sc-362323, are on our website, and the mechanism of each ingredient is well published. Formulation: Every 2 ml contains: 40 µM Trichostatin A (sc-3511) 1 mM EX-527 (sc-203044) 400 mM Nicotinamide (sc-208096). In short, each effectively inhibits HDAC and/or Sirtuins resulting in the preservation of the acetylation state of the protein. In a purified protein sample, HDAC & Sirtuin proteins may have already de-acetylated lysine residues, and sc-362323 will not re-acetylate the sample.
Answered by: Chemical Support 4
Date published: 2021-06-22
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Rated 5 out of 5 by from A combination of chemicals that was designedA combination of chemicals that was designed to preserve the acetylation state of acetyl lysine modified proteins. Tested to be suitable for use in WB, FCM and IF. Shown to inhibit HDAC's and Sirtuins in various cellular samples. -SCBT QC
Date published: 2015-03-31
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Deacetylation Inhibition Cocktail is rated 5.0 out of 5 by 1.
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