DDX28 Double Nickase Plasmid (h): sc-412609-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX28 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDX28 Double Nickase Plasmid (h) and DDX28 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDX28. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX28 Double Nickase Plasmid (h) | sc-412609-NIC | 20 µg | $410.00 |
DDX28 encodes a mitochondria-localized DEAD-box RNA helicase implicated in RNA metabolism and ribonucleoprotein remodeling within the mitochondrial compartment. It is linked to regulation of mitochondrial gene expression, including processing and translation of mitochondrial transcripts that support oxidative phosphorylation and respiratory chain function. Through its effects on mitochondrial homeostasis, DDX28 intersects with cellular energy metabolism and stress-adaptation pathways. Altered mitochondrial RNA regulation and bioenergetic imbalance are recurrent features across diverse disease contexts, making DDX28 a useful target for mechanistic studies of mitochondrial dysfunction.
DDX28 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDX28 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDX28. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDX28 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDX28-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.