DDX27 Antibody (2251C2a): sc-81074

DDX27 Antibody (2251C2a) is a mouse monoclonal IgG1 antibody that detects DDX27 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-DDX27 antibody (2251C2a) is available as non-conjugated format. DDX27, also known as probable ATP-dependent RNA helicase DDX27, is a 796 amino acid protein encoded by DDX27 gene, located on chromosome 5. DDX27 protein belongs to DEAD box helicase family, specifically DDX27/DRS1 subfamily, featuring one helicase ATP-binding domain and one helicase C-terminal domain. DDX27 protein functions as an ATP-dependent RNA helicase in nucleus, essential for RNA-related biological processes including transcription, splicing, and translation. DDX27 protein modulates RNA structure through energy derived from nucleoside triphosphate hydrolysis, maintaining proper cellular function and gene expression. DDX27 protein interacts with several specific proteins, including spliceosome components and RNA-binding proteins, highlighting DDX27′s importance in RNA metabolism regulation and potential implications in various diseases when function is disrupted.

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DDX27 Antibody (2251C2a) References:

1. The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus.  |  Doorbar, J., et al. 2000. J Virol. 74: 10081-95. PMID: 11024137
2. Verge: a novel vascular early response gene.  |  Regard, JB., et al. 2004. J Neurosci. 24: 4092-103. PMID: 15102925
3. RNA helicases: regulators of differentiation.  |  Abdelhaleem, M. 2005. Clin Biochem. 38: 499-503. PMID: 15885226
4. GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47).  |  Lee, JH., et al. 2005. Biotechnol Lett. 27: 623-8. PMID: 15977068
5. Phosphoproteome analysis of the human mitotic spindle.  |  Nousiainen, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 5391-6. PMID: 16565220
6. NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47.  |  Sekiguchi, T., et al. 2006. Nucleic Acids Res. 34: 4593-608. PMID: 16963496
7. RNA helicase, DDX27 regulates skeletal muscle growth and regeneration by modulation of translational processes.  |  Bennett, AH., et al. 2018. PLoS Genet. 14: e1007226. PMID: 29518074
8. DEAD-box helicase 27 enhances stem cell-like properties with poor prognosis in breast cancer.  |  Li, S., et al. 2021. J Transl Med. 19: 334. PMID: 34362383
9. DEAD-box Helicase 27 Promotes Hepatocellular Carcinoma Progression Through ERK Signaling.  |  Xiaoqian, W., et al. 2021. Technol Cancer Res Treat. 20: 15330338211055953. PMID: 34855554
10. METTL3-Modulated circUHRF2 Promotes Colorectal Cancer Stemness and Metastasis through Increasing DDX27 mRNA Stability by Recruiting IGF2BP1.  |  Chen, M., et al. 2023. Cancers (Basel). 15: PMID: 37370759
11. DDX27 regulates oral squamous cell carcinoma development through targeting CSE1L.  |  Li, G., et al. 2024. Life Sci. 340: 122479. PMID: 38301874
12. 18S rRNA processing requires the RNA helicase-like protein Rrp3.  |  O'Day, CL., et al. 1996. Nucleic Acids Res. 24: 3201-7. PMID: 8774901

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDX27 Antibody (2251C2a) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-DDX27 Antibody (2251C2a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDX27 Antibody (2251C2a) Specifications

Alternate Product Name: DDX27

Product Clone Name: 2251C2a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: DDX27

Epitope Species: human

Product Reactivity/Detection: DDX27

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for DDX27 Antibody (2251C2a): N/A