DDI2 Double Nickase Plasmid (h): sc-408297-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDI2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDI2 Double Nickase Plasmid (h) and DDI2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDI2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDI2 Antibody (A-3): sc-514004
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDI2 Double Nickase Plasmid (h) | sc-408297-NIC | 20 µg | $410.00 |
DDI2 (DNA damage inducible 1 homolog 2) encodes an aspartic protease–like factor that supports proteostasis and stress-adaptive transcription, most notably by enabling proteasome recovery through processing of the ER-tethered transcription factor NFE2L1/NRF1 following proteasome inhibition. Through this NRF1-dependent pathway, DDI2 helps coordinate expression of proteasome subunits and other quality-control genes, linking ubiquitin-dependent turnover with cellular responses to proteotoxic stress. DDI2 function intersects with ER-associated degradation and broader unfolded protein response signaling, influencing how cells adapt to impaired protein degradation capacity. Dysregulation of this axis is relevant to cancer cell stress tolerance and neurodegeneration-related proteostasis defects, making DDI2 a useful target for mechanistic studies of proteasome homeostasis.
DDI2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDI2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDI2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDI2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDI2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.