DDB2 Antibody (2246C4a): sc-81246

DDB2 Antibody (2246C4a) is a mouse monoclonal IgG1 kappa light chain antibody that detects the DDB2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). DDB2 (2246C4a) antibody is available as the non-conjugated anti-DDB2 antibody. DDB2, also known as Damage-Specific DNA Binding Protein 2, plays a crucial role in the cellular response to DNA damage, particularly damage induced by ultraviolet light. DDB2 forms a heterodimer with DDB1, collectively known as the DDB complex, which is essential for recognizing and binding to damaged DNA, specifically cyclobutane pyrimidine dimers. This interaction is vital for initiating nucleotide excision repair, a critical process that maintains genomic integrity and prevents mutations that can lead to cancer. Mutations in DDB2 are associated with xeroderma pigmentosum group E, a genetic disorder characterized by extreme sensitivity to sunlight and a significantly increased risk of skin cancer, underscoring DDB2′s importance in DNA repair mechanisms. Additionally, the hepatitis B virus X protein has been shown to interact with DDB1, suggesting a potential role for DDB2 in viral pathogenesis.

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DDB2 Antibody (2246C4a) References:

1. Studies of the murine DDB1 and DDB2 genes.  |  Zolezzi, F. and Linn, S. 2000. Gene. 245: 151-9. PMID: 10713455
2. Human damage-specific DNA-binding protein p48. Characterization of XPE mutations and regulation following UV irradiation.  |  Nichols, AF., et al. 2000. J Biol Chem. 275: 21422-8. PMID: 10777490
3. Dissociation of DDB1-binding and transactivation properties of the hepatitis B virus X protein.  |  Wentz, MJ., et al. 2000. Virus Res. 68: 87-92. PMID: 10930665
4. Identification of potential mRNA biomarkers in peripheral blood lymphocytes for human exposure to ionizing radiation.  |  Amundson, SA., et al. 2000. Radiat Res. 154: 342-6. PMID: 11012342
5. Damaged DNA-binding protein DDB stimulates the excision of cyclobutane pyrimidine dimers in vitro in concert with XPA and replication protein A.  |  Wakasugi, M., et al. 2001. J Biol Chem. 276: 15434-40. PMID: 11278856
6. DDB2 (damaged-DNA binding protein 2) in nucleotide excision repair and DNA damage response.  |  Stoyanova, T., et al. 2009. Cell Cycle. 8: 4067-71. PMID: 19923893
7. DDB2 (damaged-DNA binding 2) protein: a new modulator of nanomechanical properties and cell adhesion of breast cancer cells.  |  Barbieux, C., et al. 2016. Nanoscale. 8: 5268-79. PMID: 26879405
8. MBD2 facilitates tumor metastasis by mitigating DDB2 expression.  |  Zhang, L., et al. 2023. Cell Death Dis. 14: 303. PMID: 37142578
9. Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein.  |  Dualan, R., et al. 1995. Genomics. 29: 62-9. PMID: 8530102
10. Mutations specific to the xeroderma pigmentosum group E Ddb- phenotype.  |  Nichols, AF., et al. 1996. J Biol Chem. 271: 24317-20. PMID: 8798680
11. The V protein of the paramyxovirus SV5 interacts with damage-specific DNA binding protein.  |  Lin, GY., et al. 1998. Virology. 249: 189-200. PMID: 9740790
12. Refined mapping of the gene encoding the p127 kDa UV-damaged DNA-binding protein (DDB1) within 11q12-q13.1 and its exclusion in Best's vitelliform macular dystrophy.  |  Stöhr, H., et al. 1998. Eur J Hum Genet. 6: 400-5. PMID: 9781049

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDB2 Antibody (2246C4a) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-DDB2 Antibody (2246C4a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDB2 Antibody (2246C4a) Specifications

Alternate Product Name: Damage Specific DNA Binding Protein 2

Product Clone Name: 2246C4a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: DDB2

Epitope Species: human

Product Reactivity/Detection: DDB2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for DDB2 Antibody (2246C4a): N/A