Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
dCK Double Nickase Plasmid (h): sc-417715-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- dCK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- dCK Double Nickase Plasmid (h) and dCK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DCK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: dCK Antibody (H-3): sc-393099
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
dCK Double Nickase Plasmid (h) | sc-417715-NIC | 20 µg | $410.00 | |||
dCK Double Nickase Plasmid (h2) | sc-417715-NIC-2 | 20 µg | $410.00 |
Deoxycytidine kinase (DCK) encodes dCK, a cytosolic nucleoside kinase that catalyzes phosphorylation of deoxycytidine and related purine and pyrimidine deoxynucleosides, supporting deoxynucleotide salvage and dNTP homeostasis. By feeding substrates into nucleotide metabolism, dCK helps sustain DNA replication and repair and influences cellular responses to replication stress. DCK activity is closely linked to proliferation-associated metabolic remodeling and mitochondrial–nuclear nucleotide balance. Dysregulated DCK expression or function has been associated with altered nucleotide pools and has been studied in hematologic malignancies, immunobiology, and tumor metabolic adaptation, where salvage pathway dependence can shape genomic stability and cell-state transitions.
dCK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DCK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DCK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DCK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DCK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.