Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DCC Double Nickase Plasmid (h): sc-401035-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DCC Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DCC Double Nickase Plasmid (h) and DCC Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DCC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DCC Antibody (A-1): sc-515834
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DCC Double Nickase Plasmid (h) | sc-401035-NIC | 20 µg | $410.00 | |||
DCC Double Nickase Plasmid (h2) | sc-401035-NIC-2 | 20 µg | $410.00 |
DCC (deleted in colorectal carcinoma) encodes a netrin-1 receptor in the immunoglobulin superfamily that regulates axon guidance, neuronal migration, and cytoskeletal remodeling through pathways including Rho family GTPase signaling and focal adhesion dynamics. In the absence of ligand, DCC can function as a dependence receptor that influences apoptosis and cell survival, linking extracellular cue sensing to intracellular signaling decisions. Beyond neurodevelopment, DCC contributes to cell adhesion and directed migration in diverse tissues, and altered DCC expression or genomic disruption has been associated with tumor progression, invasion phenotypes, and neurodevelopmental abnormalities. These features make DCC a useful target for mechanistic studies of guidance cue signaling, morphogenesis, and disease-relevant changes in cell motility and survival programs.
DCC Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DCC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DCC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DCC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DCC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.