DAT/Dopamine Transporter/SLC6A3 Antibody (6-5G10): sc-32258

DAT Antibody (6-5G10) is a rat monoclonal IgG antibody that detects DAT (Dopamine Transporter) from mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-DAT antibody (6-5G10) is available in non-conjugated form and conjugated to agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® dyes. The dopamine transporter (DAT), a member of the solute carrier family 6, regulates dopamine levels in the synaptic cleft by facilitating dopamine reuptake from extracellular space into presynaptic neurons. This function maintains dopaminergic signaling, essential for mood regulation, reward, and motor control. DAT dysfunction has been linked to various neuropsychiatric disorders, including depression, schizophrenia, and attention deficit hyperactivity disorder (ADHD), highlighting DAT′s importance in health and disease. Additionally, DAT′s ability to transport neurotoxins contributes to dopaminergic neuron vulnerability in conditions like Parkinson′s disease. Studying DAT dynamics and protein interactions may reveal new therapeutic approaches for these disorders.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DAT/Dopamine Transporter/SLC6A3 Antibody (6-5G10) References:

1. Multiple second messenger pathways of alpha-adrenergic receptor subtypes expressed in eukaryotic cells.  |  Cotecchia, S., et al. 1990. J Biol Chem. 265: 63-9. PMID: 2152928
2. Dopamine Transporter (DaT) Scan Utilization in a Movement Disorder Center.  |  Oravivattanakul, S., et al. 2016. Mov Disord Clin Pract. 3: 31-35. PMID: 30363515
3. Multimodal dopamine transporter (DAT) imaging and magnetic resonance imaging (MRI) to characterise early Parkinson's disease.  |  Porter, E., et al. 2020. Parkinsonism Relat Disord. 79: 26-33. PMID: 32861103
4. Dopamine transporter (DAT) imaging in Parkinson's disease and related disorders.  |  Brücke, T. and Brücke, C. 2022. J Neural Transm (Vienna). 129: 581-594. PMID: 34910248
5. Generation of Dopamine Transporter (DAT)-mCherry Knock-in Rats by CRISPR-Cas9 Genome Editing.  |  Matsumoto, N., et al. 2024. Biol Pharm Bull. 47: 394-398. PMID: 38325828
6. Risperidone Decreases Expression of Serotonin Receptor-2A (5-HT2A) and Serotonin Transporter (SERT) but Not Dopamine Receptors and Dopamine Transporter (DAT) in PBMCs from Patients with Schizophrenia.  |  Alvarez-Herrera, S., et al. 2024. Pharmaceuticals (Basel). 17: PMID: 38399382
7. Characterizing enteric neurons in dopamine transporter (DAT)-Cre reporter mice reveals dopaminergic subtypes with dual-transmitter content.  |  Recinto, SJ., et al. 2024. Eur J Neurosci. 59: 2465-2482. PMID: 38487941
8. Agonist-induced desensitization of dopamine D1 receptor-stimulated adenylyl cyclase activity is temporally and biochemically separated from D1 receptor internalization.  |  Ng, GY., et al. 1995. Proc Natl Acad Sci U S A. 92: 10157-61. PMID: 7479745
9. The conserved seven-transmembrane sequence NP(X)2,3Y of the G-protein-coupled receptor superfamily regulates multiple properties of the beta 2-adrenergic receptor.  |  Barak, LS., et al. 1995. Biochemistry. 34: 15407-14. PMID: 7492540
10. Molecular and chemical neuropharmacology of dopamine receptor subtypes.  |  Ogawa, N. 1995. Acta Med Okayama. 49: 1-11. PMID: 7762403
11. The D2 dopamine receptor isoforms signal through distinct Gi alpha proteins to inhibit adenylyl cyclase. A study with site-directed mutant Gi alpha proteins.  |  Senogles, SE. 1994. J Biol Chem. 269: 23120-7. PMID: 7916015
12. The dopamine transporter is localized to dendritic and axonal plasma membranes of nigrostriatal dopaminergic neurons.  |  Nirenberg, MJ., et al. 1996. J Neurosci. 16: 436-47. PMID: 8551328

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DAT/Dopamine Transporter/SLC6A3 Antibody (6-5G10) has 60 citations in a variety of scientific publications.

Primary antibodies like Anti-DAT/Dopamine Transporter/SLC6A3 Antibody (6-5G10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DAT/Dopamine Transporter/SLC6A3 Antibody (6-5G10) Specifications

Alternate Product Name: Dopamine Active Transporter (DAT)

Product Clone Name: 6-5G10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids: 1-66

Epitope Target: DAT

Epitope Species: human

Product Reactivity/Detection: DAT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 60 citations in various scientific publications

Available Conjugates/Forms for DAT/Dopamine Transporter/SLC6A3 Antibody (6-5G10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin