DaRS Antibody (14S6): sc-100986

DaRS Antibody (14S6) is a mouse monoclonal IgG2a antibody that detects DaRS in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). DaRS, or aspartyl-tRNA synthetase, is a crucial enzyme in the protein biosynthesis pathway, responsible for the specific aminoacylation of tRNA with aspartate, which is essential for the accurate translation of genetic information into proteins. DaRS localization in the cytoplasm is particularly important, as this is where the ribosomal machinery operates, and any dysfunction can lead to errors in protein synthesis, potentially resulting in cellular stress or disease. Additionally, DaRS is part of a multisubunit complex that facilitates the transfer of Asp-tRNA to elongation factor alpha 1, highlighting its role in ensuring the efficiency and fidelity of protein synthesis. The N-terminal extension of DaRS, which is unique to vertebrates, contains a putative amphiphilic helix that acts as a regulatory switch, enhancing the enzyme′s catalytic function by stabilizing the Asp-tRNA interaction. With dual phosphorylation sites and ability to form homodimers, DaRS is a dynamic protein that plays a pivotal role in cellular metabolism and the maintenance of protein homeostasis.

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DaRS Antibody (14S6) References:

1. Development and validation of the Dimensional Anhedonia Rating Scale (DARS) in a community sample and individuals with major depression.  |  Rizvi, SJ., et al. 2015. Psychiatry Res. 229: 109-19. PMID: 26250147
2. [DARS mutations responsible for hypomyelination with brain stem and spinal cord involvement and leg spasticity: report of two cases and review of literature].  |  Zhang, J., et al. 2018. Zhonghua Er Ke Za Zhi. 56: 211-215. PMID: 29518832
3. LncRNA DARS-AS1 aggravates the growth and metastasis of hepatocellular carcinoma via regulating the miR-3200-5p-Cytoskeleton associated protein 2 (CKAP2) axis.  |  Feng, Y., et al. 2021. Bioengineered. 12: 8217-8232. PMID: 34596006
4. lncRNA DARS-AS1 Promoted Osteosarcoma Progression through Regulating miR-532-3p/CCR7.  |  Xue, Y., et al. 2022. Dis Markers. 2022: 4660217. PMID: 35422889
5. DARS-AS1 recruits METTL3/METTL14 to bind and enhance DARS mRNA m6A modification and translation for cytoprotective autophagy in cervical cancer.  |  Shen, W., et al. 2022. RNA Biol. 19: 751-763. PMID: 35638109
6. lncRNA DARS-AS1 Modulates TSPAN1-Mediated ITGA2 Hypomethylation by Interaction with miR-194-5p Thus Promoting Ovarian Cancer Progression.  |  Li, J., et al. 2022. Stem Cells Int. 2022: 4041550. PMID: 36187230
7. DARS-AS1 modulates cell proliferation and migration of gastric cancer cells by regulating miR-330-3p/NAT10 axis.  |  Du, C., et al. 2022. Open Med (Wars). 17: 2036-2045. PMID: 36568518
8. DARS-AS1: A Vital Oncogenic LncRNA Regulator with Potential for Cancer Prognosis and Therapy.  |  Shu, J., et al. 2024. Int J Med Sci. 21: 571-582. PMID: 38322590
9. LncRNA DARS-AS1 in human cancers: A comprehensive review of its potency as a biomarker and therapeutic target.  |  Dang, Q. 2024. Gene. 923: 148566. PMID: 38762015
10. DARS expression in BCR/ABL1-negative myeloproliferative neoplasms and its association with the immune microenvironment.  |  Xiong, H., et al. 2024. Sci Rep. 14: 16711. PMID: 39030308

The preferred DaRS antibody is DaRS Antibody (H-3): sc-393275.