DARPP-32 Antibody (15) recommended for detection of DARPP-32 of mouse, rat and human origin by WB, IP and IF

DARPP-32 Antibody (15): sc-135877

DARPP-32 Antibody (15) is rated 4.0 out of 5 by 2.
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Datasheets

    • DARPP-32 Antibody (15) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 70-181 of DARPP-32 of human origin
    • recommended for detection of DARPP-32 of mouse, rat and human origin by WB, IP and IF
    • See DARPP-32 (H-3): sc-271111 for DARPP-32 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using DARPP-32 (15): sc-135877 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good strong band obtained in WB in rat brain Good strong band obtained in WB in rat brain tissue extract. -SCBT QC
Date published: 2015-03-25
Rated 4 out of 5 by from WB WB, IF publishable data with human gastric cancer celllines - SCBT Publication Review
Date published: 2013-07-19
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